0.5M EDTA, pH 8.0
0.5M EDTA, pH 8.0

EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical, and a sequestrant. Suitable for use in molecular biology applications. EDTA is used extensively as chelator of divalent cations such as Ca2+. Useful in molecular biology applications to minimize metal ion contaminants. Also used to facilitate enzymatic reactions that could be inhibited by heavy metal traces. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

 
Name Cat# Description Price Qty
0.5M EDTA, pH 8.0
5EP8-100
400 ml
$77.25
0.5M EDTA, pH 8.0
5EP8-200
1 L
$154.50
0.5M EDTA, pH 8.0
5EP8-OEM
OEM
please inquire

Description:
EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical, and a sequestrant. Suitable for use in molecular biology applications. EDTA is used extensively as chelator of divalent cations such as Ca2+. Useful in molecular biology applications to minimize metal ion contaminants. Also used to facilitate enzymatic reactions that could be inhibited by heavy metal traces. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Applications:

  • Termination of metal dependent enzymatic reactions
  • Protection of DNA against metal dependent nucleases

Molecular Weight: 358.19

Molecular Formula: C10H13N2O8Na3

Recommended Storage Condition: Room Temperature

References:
1. Child, G.P. 1951. Science. 114: 466-467. PMID: 14892768
2. Friess, E.G., et al. 1954. Arch Biochem. 53: 311-313. PMID: 13208306
3. Sapeika, N. 1955. S. Afr. Med. J. 29: 137-139. PMID: 14358876
4. Sheppard, R.L., et al. 1997. Anal. Chem. 69: 477A-480A. PMID: 9253241
5. Lanigan, R.S. and Yamarik, T.A. 2002. Int. J. Toxicol. 21: 95-142. PMID: 12396676

MSDS & Certificates

The Q&A for this product will be available soon.
Customized Competent <i>E. coli</i>
BL21(DE3) Competent <i>E. coli</i>
HB101 Competent <i>E. coli</i>
Customized Competent E. coli BL21(DE3) Competent E. coli HB101 Competent E. coli
$550.00
$110.00
$110.00
 
BL21(DE3)pLysS Competent <i>E. coli</i>
Dh5-Alpha Competent  <em>E. coli </em>
T4 Endonuclease VII
BL21(DE3)pLysS Competent E. coli Dh5-Alpha Competent E. coli T4 Endonuclease VII
$110.00
$110.00
$200.00
 
Exonuclease I (<i>E. coli</i>)
<em>Taq</em> DNA Polymerase
Brij-35 (30% Solution)
Exonuclease I (E. coli) Taq DNA Polymerase Brij-35 (30% Solution)
$100.00
$100.00
$66.00