| 1 kb DNA Ladder- premixed Description The 1 Kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 500 bp to 9 kb.The ladder may be radioactively labeled by one of the following methods: (i) Partial exonucleolytic degradation and resynthesis with T4 DNA polymerase. This method is preferred because higher specific activity is achieved with less 32P input; (ii) Labeling the ends with T4 polynucleotide kinase; (iii) Filling in the recessed ends with E. coli DNA polymerase I or the large fragment of DNA polymerase I. This ladder is premixed with loading dyes for direct gel electrophoresis. Application Determination of the molecular weight of nucleic acids Concentration and Usage: Conc. 0.1 μg/μl and 5 μl per lane Storage Store at room temperature. Stability Store at room temperature. Recommended Storage Condition Please store at -20°C. |
