MCLAB Products

2X HotSybr Real-time PCR Kit

Description

4x1.25ml for 200 reactions. Designed for all kinds of real-time PCR machines.

*Note:

Name	Cat#	Description	Price	Qty
2X HotSybr Real-time PCR Kit	HSM400	Regular level of ROX, for Real-time PCR Machines ABI 7000, 7300, 7700, 7900, 200 rnx, 4x1.25ml	$255.00
2X HotSybr Real-time PCR Kit	HSM400LR	Low level of ROX, for Real-time PCR Machines ABI 7500, Stratagene Mx 3000P, Mx 3005P, 200 rnx, 4x1.25ml	$255.00
2X HotSybr Real-time PCR Kit	HSM400RF	ROX Free, for Real-time PCR Machines BioRad iCycler MiniOpticon, Opticon 2, Chromo4, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo4; Corbett Roto-gene 3000, 6000, 200 rnx, 4x1.25ml	$255.00

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2X HotSybr Real-time PCR Kit

Catalog No.	Document
HSM400	HSM400
HSM400LR	HSM400RF
HSM400RF	HSM400LR
Related Products: HTRT, HTP

For Research Use Only.

PRODUCT
This is a high performance real-time PCR reagent. It utilizes our own proprietary quantitative PCR technology.

ADVANTAGES
1. This product is similar to ABI's Power SYBR® Green PCR Master Mix (CAT# 4367659) but priced 25% less!

2. MCLAB's 2X HotSybr PCR Reaction Mix products cuts 1/2 total reaction time.

Normally the total reaction time is 4350 sec: 95°C, 10min => (95°C, 15sec => 60°60sec) x 50
For MCLAB's 2X HotSybr PCR Reaction Mix, the total reaction time is 2350 sec: 95°C, 10min => (95°C, 5sec => 60°, 30sec) x 50

3. MCLAB's One-step RT-PCR products are faster.

4. MCLAB's 2X HoTaq PCR Reaction Mix products are superior in amplifying difficult templates.

This is the amplification of GPIIB gene (70% G+C) .
10 ~ 10K copies from 30pg human genomic DNA have been detected.

Applications
Probe based quantitative PCR including DNA quantification, 2-step RT PCR, SNP analysis, etc.

Primer and probe design
Appropriate software, such as ABI Primer Express^TM, should be used.Recommended reaction conditions:
95Â°C, 10min -> (95Â°C, 5sec. -> 60Â°C, 30sec.) for 50 cycles -> melting curve.

Recommended storage condition
-20^oC

Notes
To achieve accurate quantification, it is highly recommended to (1) do replicates; (2) reduce pipetting error; (3) primer concentration from 100nM to 300nM; (4) run melting curve following amplification cycles.

Reference

Higuchi R, Dollinger G, Walsh PS, Griffith R; Bio/Technology 10: 413â417, 1992

FOR RESEARCH USE ONLY.

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2X HotSybr Real-time PCR Kit

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