5’ Adenylation (rApp Modification) Service
5’ Adenylation (rApp Modification) Service

The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.

 
Name Cat# Description Price Qty
5’ Adenylation (rApp Modification)
5APP-100
100 nmole DNA Oligo
$400.00
5’ Adenylation (rApp Modification)
5APP-200
250 nmole DNA Oligo
$500.00
5’ Adenylation (rApp Modification)
5APP-300
1 µmole DNA Oligo
$1000.00
5’ Adenylation (rApp Modification)
5APP-400
5 µmole DNA Oligo
$4000.00
5’ Adenylation (rApp Modification)
5APP-500
10 µmole DNA Oligo
$5000.00

Description:
The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.

Application:
5’ adenylated oligos are used in sequence detection, sequence capture, sequencing by ligation and attachment of adapters (e.g. RNA library preparation for NGS and miRNA library construction), etc.

Starting material requirement:
1)    Order oligos with 5’ phosphate.
2)    To prevent a 5’ adenylated oligo from self-ligating, a blocking group is required on the 3’ end, such as a 2’, 3’-Dideoxynucleotide or 3’ (C3) Propyl Spacer.
3)    5’ adenylated oligos require an extra purification after the adenylation step and are not compatible with heat-sensitive modifications. Prices are valid for oligonucleotides up to 30 bases. For longer oligos, please inquire for a custom quotation.
Adenylation

There is no documents for this product.Will be available soon.
The Q&A for this product will be available soon.
HB101 Competent <i>E. coli</i>
JM109 Competent <i>E. coli</i>
T4 Endonuclease VII
HB101 Competent E. coli JM109 Competent E. coli T4 Endonuclease VII
$110.00
$110.00
$200.00
 
BL21(DE3)pLysS Competent <i>E. coli</i>
2X HotStart PCR Master Mix
Phi29 DNA Polymerase
BL21(DE3)pLysS Competent E. coli 2X HotStart PCR Master Mix Phi29 DNA Polymerase
$110.00
$60.00
$100.00
 
Exonuclease I (<i>E. coli</i>)
<em>Taq</em> DNA Polymerase
Brij-35 (30% Solution)
Exonuclease I (E. coli) Taq DNA Polymerase Brij-35 (30% Solution)
$100.00
$100.00
$66.00