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TWEEN 20
TWEEN 20

TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v. These buffers are used for washes between each immunoreactions, to remove unbound immunologicals, and eventually for incubation solutions of immunoreagents (labeled antibodies) to reduce unspecific background.
 
Name Cat# Description Price Qty
TWEEN 20
50T020-1L
50%, 1L
$60.00
TWEEN 20
50T020-1GAL
50%, 1GAL
$150.00

Description:
TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v. These buffers are used for washes between each immunoreactions, to remove unbound immunologicals, and eventually for incubation solutions of immunoreagents (labeled antibodies) to reduce unspecific background.

Applications:

  • to saturate binding sites on surfaces (i.e. to coat polystyrene microplates, generally combined to proteins such as BSA).
  • to stabilize proteins purified in protein derivative (PPD) solution used in skin testing for tuberculosis exposure
  • as a solubilizing agent of membrane proteins
  • for lysing mammalian cells, at a concentration of 0.05% to 0.5% v/v, generally combined to other detergents, salts and additives.

Recommended Storage Condition: room temperature

References:

  1. Ayorinde FO, Gelain SV, Johnson JH Jr, Wan LW. (2000). "Analysis of some commercial polysorbate formulations using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry". Rapid Communications in Mass Spectrometry 14 (22): 2116–2124. doi:10.1002/1097-0231(20001130)14:22<2116::AID-RCM142>3.0.CO;2-1. PMID 11114018.
  2. Joint FAO/WHO Expert Committee on Food Additives (1974). "Toxicological evaluation of some food additives including anticaking agents, antimicrobials, antioxidants, emulsifiers and thickening agents". WHO Food Additives Series No. 5. World Health Organization.
  3. Chunhee Kim, You-Lo Hsieh (2001). "Wetting and absorbency of nonionic surfactant solutions on cotton fabrics". Colloids and Surfaces A 187: 385–397. doi:10.1016/S0927-7757(01)00653-7.
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