ATP Sulfurylase Yeast

Description:
Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containg 511 amino acids and having a Mw of 57.7 kDa. Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi.

Adenosine 5 Triphosphate Sulfurylase is purified by proprietary chromatographic techniques.

Applications:

• synthesizes adenosine 5’-sul-phatophosphate from ATP and inorganic SO42-
• catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate (PPi)

Source:
Escherichia Coli

Unit Definition:
One unit is the amount of enzyme which incorporates 1 pmol AMP into acid-insoluble material at 37°C in 1 min.

Unit Assay Conditions:
115 mM Tris-HCl (pH 8.0), 0.58 mM b-NADP, 2.4 mM Mg acetate, 34 mM D-glucose, 0.3 mM adenosine 5´-phosphosulfate, 3.4 mM pyrophosphate, 0.75 units/ml hexokinase and 0.5 units/ml glucose 6-phosphate dehydrogenase.