ATP Sulfurylase Yeast
ATP Sulfurylase Yeast

Adenosine 5' Triphosphate Sulfurylase is purified from E.coli containing Yeast adenosine 5' Triphosphate Sulfurylase gene.
 
Name Cat# Description Price Qty
ATP sulfurylase Yeast
ATPSY-100
200 µl, 2 mg/ml
$278.10
ATP sulfurylase Yeast
ATPSY-200
500 µl, 2 mg/ml
$412.00
ATP sulfurylase Yeast
ATPSY-OEM
Any Size
please inquire

Description:
Adenosine 5'-Triphosphate Sulfurylase Yeast Recombinant produced in E. coli is a non-glycosylated, polypeptide chain containing 511 amino acids and having a MW of 57.7 kDa. Adenosine 5'-Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5'-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi. Adenosine 5'-Triphosphate Sulfurylase is purified by proprietary chromatographic techniques.

Application:
- Synthesizes adenosine 5'-sulphatophosphate from ATP and inorganic SO42-
- Catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5'-phosphosulfate (APS) and pyrophosphate (PPi)

Source:
Escherichia coli containing Yeast adenosine 5'-Triphosphate Sulfurylase gene

Unit Definition:
One unit is the amount of enzyme which incorporates 1 pmol AMP into acid-insoluble material at 37°C in 1 minute.

Unit Assay Conditions:
115 mM Tris-HCl(pH 8.0), 0.58 mM ß-NADP, 2.4 mM Mg acetate, 34 mM D-glucose, 0.3 mM adenosine 5'-phosphosulfate, 3.4 mM pyrophosphate, 0.75 units/ml hexokinase and 0.5 units/ml glucose 6-phosphate dehydrogenase.
 

Recommended Storage Condition: -20ºCDescription:
Adenosine 5'-Triphosphate Sulfurylase Yeast Recombinant produced in E. coli is a non-glycosylated, polypeptide chain containing 511 amino acids and having a MW of 57.7 kDa. Adenosine 5'-Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5'-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi. Adenosine 5'-Triphosphate Sulfurylase is purified by proprietary chromatographic techniques.

Application:
- Synthesizes adenosine 5'-sulphatophosphate from ATP and inorganic SO42-
- Catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5'-phosphosulfate (APS) and pyrophosphate (PPi)

Source:
Escherichia coli containing Yeast adenosine 5'-Triphosphate Sulfurylase gene

Unit Definition:
One unit will produce 1.0 μmole of ATP from APS and inorganic pyrophosphate per min at pH 8.0 at 30°C.

Activity: Measured by its ability to sulfurylate ATP. The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Unit Assay Conditions:
115 mM Tris-HCl(pH 8.0), 0.58 mM ß-NADP, 2.4 mM Mg acetate, 34 mM D-glucose, 0.3 mM adenosine 5'-phosphosulfate, 3.4 mM pyrophosphate, 0.75 units/ml hexokinase and 0.5 units/ml glucose 6-phosphate dehydrogenase.

Recommended Storage Condition: -20ºC

 

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