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Ab Cloning 5'-RACE Kit
Ab Cloning 5'-RACE Kit

The Ab cloning RACE kit provides a novel method for performing both 5' and 3’ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning both heavy and light chains of human and mouse antibodies.

 
Name Cat# Description Price Qty
Ab cloning 5'-RACE Kit
ABC5-100
10 rxn
$429.00
Ab cloning 5'-RACE Kit
ABC5-200
20 rxn
$649.00
Ab cloning 5'-RACE Kit
ABC5-OEM
any size
please inquire

General Description
The Ab cloning RACE kit provides a novel method for performing both 5' and 3’ rapid amplifications of cDNA ends (RACE) with optimized primers for cloning both heavy and light chains of human and mouse antibodies, respectively. MCLAB's SmartRT reverse transcriptase is an engineered MMLV RT with increased thermostability, improved cDNA synthesis ability; and a reduced RNase H activity. The enzyme also has a terminal transferase activity.  This tailing activity (terminal transferase activity) of the SmartRT reverse transcriptase allows to synthesize complete cDNA by SMART (Switching Mechanism At 5' end of RNA Transcript) cDNA synthesis technology. With 3'-5' extra nuclear acids added to the 3' end of the first-strand cDNA, 5’ SMART universal oligo contains terminal complementation to nuclear acids at 3' end of the first-strand cDNA can be annealed to first-strand cDNA tail and serves as an extended template for RT. The switch of template from mRNA to 5’ SMART universal oligo produces a complete cDNA copy of transcript RNA with 5’ SMART universal oligo at the end. The cDNA transcript from smarter RT can be used for directly in 5’ and 3’ race with MCLAB I-5™ 2X High-Fidelity Master Mix. Our 5’/3’ Race kit is optimized for antibody cloning. It also can be used for any gene of interest. 

Features

  • Specific full length cDNA enrichment technique
  • Optimized primer set for full length antibody sequencing
  • Simplified protocol without 5’ end adaptor-ligation step

Application
Antibody full length sequencing

Components

Components

Cap Color

Volume (10 rxn)

Volume (20 rxn)

Oligo dT Primer

Orange

10 µl

20 µl

5X RT Reaction Buffer

Green

40 µl

80 µl

dNTP

Yellow

10 µl

20 µl

DTT

Yellow

5 µl

10 µl

SmartRT™ reverse transcriptase

Clear

20 µl

40 µl

5’ Universal Primer

Red

10 µl

20 µl

3’ IgG Primer

Red

10 µl

20 µl

3’ IgM Primer

Red

10 µl

20 µl

3’ IgE Primer

Red

10 µl

20 µl

3’ Kappa Chain Primer

Red

10 µl

20 µl

3’ Lambda Chain Primer

Red

10 µl

20 µl

I-5™ 2X High-Fidelity Master Mix

Purple

100 µl

200 µl

RNAse Inhibitor

Blue

5 µl

10 µl

Storage Condition for above components: -20 °C

Cap Finder (Store at -80 °C)

Clear

10 µl

20 µl

 

Recommended Storage Condition
-20°C

Shipping Condition
 dry ice

 

Manual & Protocols



MSDS & Certificates

RNAse Inhibitor
Figure 1.The RACE ready cDNA was synthesized from 1ug of total RNA from hybridoma cells using the Ab cloning RACE kit. 4 ul of cDNA were used for 5’ and 3’ RACE PCR. The gel image shows the amplified antibody heavy chain and light chain cDNA. 

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