Acetylated Bovine Serum Albumin (BSA)

Description:
Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is normally added to restriction digests.

Application:

• Used as a blocking agent in Northern, Southern and dot blot hybridizations
• Added to buffers for nick translation, polymerase reactions and ligations
• Common additive for PCR amplifications, footprinting and gel shift assays
• Helps stabilize some proteins during incubation
• Enhances enzyme activity in restriction digests

Concentration:
20 mg/ml

Recommended Storage Conditions:
BSA should be stored at –20°C. Do not store in a frost-free freezer.