Antibody CDR Region (Complementarity Determining Region) determination

Descriptions:
Immunoglobulins are composed of polymorphic heavy and light chains. The idiotypic variability is related to the diversity of the antigen binding site and in particular to the hyper variable domains called complementarity-detering regions (CDRs). There are 6 CDRs in both variable regions of light (VL) and heavy chains (VH) with background variability on each side of the CDRs. Antibodies of different specificities can assemble identical VL domains with different VH domains. The framework sequences between CDRs can be similar or identical.
MCLAB offers a fast and professional service to determine your valuable antibody CDRs via cDNA cloning and sequencing. We can determine any antibody producing cell line tailored to your requirements.
MCLAB has extensive experience in antibody CDR determination from cDNA. Sequences are compiled and aligned based on bi-directional sequencing of multiple independent clones, then determine the CDRs by our proprietary bioinformatics tools.

Features:

1. The lowest price and the fastest service in the market.
2. Relability and productivity based on technological experience.

Starting Materials:
A pellet of snap frozen cells (1x105~1x107 cells) or B-cell line expressing your antibody.

Technical Summary:
Stage 1: Total RNA extraction from the cell pellet
Stage 2: Reverse transcription
Stage 3: PCR or 5’ RACE amplification of heavy and light chains
Stage 4: Cloning into a standard sequencing vector and sequencing
Stage 5: Determination of the peptides corresponding to the CDRs
Stage 6: Final Report, including sequence alignments of the heavy and light chains and the peptide sequences corresponding to the CDRs, to client.