MCLAB Products

BDX64 (BigDye Enhancing Buffer)

Description

MCLABs BDX64 is a BigDye enhancing buffer. It has the same ion strength as the BigDye premix 3.1 and 1.1.



MSDS

*Note:

Name	Cat#	Description	Price	Qty
BDX64 Buffer	BDX-100	2 x1.25 ml	$210.00

Ask a Question/Quote about this product

BDX64

Catalog No.	Size	Price
BDX-OEM	Any size	Please inquire

For Research Use Only.

Descriptions

MCLABs BDX64 is a BigDye enhancing buffer. It has the same ion strength as the BigDye premix 3.1 and 1.1.

Up to 64 (0.125 µl bigdye in 10 µl reaction) or more fold dilutions by combing with the 5X dilution buffer.
Enhances the polymerase activity and reduces the extension time from 4 to 1 minute.
Reduces signal decline rate and results in even peak distribution.
Optimizes for use with BigDye Chemistry (ABI) on 377, 3100, 3130/3130XL & 3730/3730XL.

An Application Example (64 fold)

BDX64: 0.875 µl
BigDye3.1: 0.125 µl
5X dilution buffer: 1.5 µl (MCLABs catalog # is SBUF-100 for this product)
Template: 100 ng
Primer: 3.2 pmole
H₂O: 10 µl

Recommended Cycle Conditions
96°C, 3 min. + 30X (96°C, 10 sec. 50°C, 5 sec. 60°C, 2 min.)

Storage: Store at 20°C

Sequencing Examples

1. Dilution fold 32X:
1.5µl 5X dilution buffer + 0.750µl BDX64 buffer + 0.250µl Bigdye" Terminator 3.1, total reaction volume 10µl

2. Dilution fold 64X:
1.5µl 5X dilution buffer + 0.875µl BDX64 buffer + 0.125µl Bigdye" Terminator 3.1, total reaction volume 10µl

3. Dilution fold 128X:
1.5µl 5X dilution buffer + 0.937µl BDX64 buffer + 0.063µl Bigdye" Terminator 3.1, total reaction volume 10µl

4. Dilution fold 256X:
1.5µl 5X dilution buffer + 0.969µl BDX64 buffer + 0.031µl Bigdye" Terminator 3.1, total reaction volume 10µl

Signal Distributions: before and after BDX64 was added

Your name:	*
Your email:	*
Recipient's email:	*
Add a personal message


IgG purification using Protein G	Monoclonal Antibody	T4 RNA Ligase 2 (dsRNA Ligase)
$198.00	$0.90	$35.00


Recombinant Protein G, Agarose	Recombinant Protein A	IgG Purification using Protein A
$360.00		$472.00


T4 RNA Ligase 2 (truncated) (RNL2)	HpyA V	Recombinant Protein G, Biotinylated
$120.00	$32.00	$70.00

Your name:	*
Your message:	*
Image verification
Type the characters you see in the picture:
	*

BDX64 (BigDye Enhancing Buffer)

Product rating

Customer Reviews

Add your review here

Your cart

Recently Viewed

Special

Help

News