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BDX64 (BigDye<sup></sup> Enhancing Buffer)
BDX64 (BigDye Enhancing Buffer)

MCLAB's BDX64 is a BigDye® enhancing buffer, it is used for DNA sequencing. BDX64 buffer has the same ion strength as the BigDye® premix 3.1 and 1.1.

 
Name Cat# Description Price Qty
BDX64 Buffer
BDX-100
2 x1.25 ml
$263.00
BDX64 Buffer
BDX-OEM
Any size
please inquire

Description:
MCLAB's BDX64 is a BigDye® enhancing buffer, it is used for DNA sequencing. BDX64 buffer has the same ion strength as the BigDye® premix 3.1 and 1.1.
Up to 64 (0.12 µl bigdye in 10µl reaction) or more fold dilutions by combining with the 5x dilution buffer
Enhances the polymerase activity and reduces the extension time from 4 to 1 minute.
Reduces the signal decline rate and results in even peak distributions.
Optimizes for using with BigDye® Chemistry (ABI) on 310, 3100, 3130/3130xl & 3730/3730xl.
 

Application:

Component

Volume

Volume

   BigDye® 3.1

0.25 µl

0.5 µl

   5x buffer (cat# SBUF-100)

0.875 µl

0.75 µl

   BDX64 buffer

2 µl

2 µl

   Template

100 ng

100 ng

   Primer

3.2 pmol

3.2 pmol

   Add H2O to 10 µl

   

 

Sequencing Examples:
1. Dilution fold 32X:


2. Dilution fold 64X:


3. Dilution fold 128X:


4. Dilution fold 256X:


Signal Distributions: before and after BDX64 was added


Recommended Cycle Conditions:
96°C, 3 minutes. + 30x (96°C, 10 seconds. 50°C, 5 seconds. 60°C, 2 minutes.)

Recommended Storage Condition: -20°C

COA ( Certificate of Analysis )



MSDS & Certificates

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