BL21(DE3)pLysS Competent E. Coli

BL21(DE3)pLysS Competent E. Coli
Description

High yields, high efficiency, no heat shock necessary.
 
Name Cat# Description Price Qty
BL21(DE3)pLysS
BP-100
10x100μl (10 tubes), >1x10⁸
$110.00
BL21(DE3)pLysS
BP-196
96x50μl (96-tube rack), >1x10⁸
$320.00

Description
High yields, high efficiency, no heat shock necessary.

Application

  • Ideal for expressing proteins that are toxic to E.Coli.
  • BL21(DE3)pLysS cells carry the lambda DE3 lysogen. In addition, L21(DE3)pLysS cells contain the pLysS plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. This provides tight control of T7 RNA polymerase,which is necessary when the recombinant protein to be expressed is toxic.

Recommended torage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions

  • Mix DNA with competent cell
  • Let stand on bench for 5 minutes
  • Direct plate

Genotype
F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR)

Reference
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1x108

Blue-Whiet Screening:
No

Strain:
B

Reduces Recombination:
No

Cloning Methylated DNA:
No

Improves Plasmid Quality:
No

Preparing Unmethylated DNA:
Not Suitable

T1 Phage Resistant:
No

RecA Deficient:
No



COA ( Certificate of Analysis )



MSDS & Certificates

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