Description:
Benzonase is a genetically engineered endonuclease from Serratia marcescens(1–2).The three-dimensional crystal structure of Serratia marcescens (Sm) nuclease has been refined at 1.7 Å resolution to the R-factor of 17.3% and R-free of 22.2%. The final model consists of 3678 non-hydrogen atoms and 443 water molecules. The analysis of the secondary and the tertiary structures of the Sm nuclease suggests a topology which reveals essential inner symmetry in all the three layers forming the monomer. We propose the plausible mechanism of its action based on a concerted participation of the catalytically important amino acid residues of the enzyme active site.
Applications:
-
Removal of nucleic acid contaminants from recombinant protein preparations
-
Viscosity reduction in protein extracts
-
Sample preparation for 2D gel electrophoresis
Source:
Genetically engineered endonuclease from Serratia marcescens produced and purified from an E. coli strain
Unit Definition:
One unit of Benzonase Nuclease id defined as the amount of enzyme that caused a ΔA260 of 1.0 in 30
Recommended Storage Condition:
The enzyme is stable for 2 years when stored at -20°C.
References:
-
Eaves, G.N. and Jeffries, C.D. (1963) J. Bacteriol. 85, 273–278.
-
Nestle, M. and Roberts, W.K. (1969) J. Biol. Chem. 244, 5213–5218.
|
