Bgl II

Recognition site:
A^GATCT

Source:
Purified from Bacillus globigii.

Storage buffer:
50 mM Tris-HCl (pH 7.4), 150 mM KCl, 1mM EDTA, 0.5 mg.ml BSA, 50% (v/v) glycerol.

Reaction conditions:
REACT® 3 Buffer at 37°C.

Typical ligation/recut assay results
Bgl II-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Bgl II.

Recommended storage conditions:
-20°C.

Comments:
Does not cleave DNA when the C residue is 5-methylcytosine, but cleaves DNA when the 3’A residue is N6 –methyladenine. Resistant to heat inactivation (10 min, 65°C).