BigDye® Terminator 5X Sequencing Buffer

BigDye® Terminator 5X Sequencing Buffer
Description

The BigDye® Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or read length. Its buffer system enables to use less dye terminator mix.
           
MSDS          
MSDS          
 
Name Cat# Description Price Qty
BigDye Terminator 5X Sequencing Buffer
SBUF-100
1 ml
$25.00
BigDye Terminator 5X Sequencing Buffer
SBUF-110
28 ml
$470.00
BigDye Terminator 5X Sequencing Buffer
SBUF-120
233 ml
$3495.00
BigDye Terminator 5X Sequencing Buffer
SBUF-OEM
Any size
please inquire
Description:
The BigDye® Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or read length. Its buffer system enables to use less dye terminator mix.

Application:
DNA sequencing
Fragment analysis

Recommended Storage Conditions: 4°C

Recommended Protocol:
Prepare sequencing reactions (10µl rxn) according to the following:
- Add dye terminator mix 0.1-0.2µl
- Add 5X Sequencing Buffer 2µl
- Add template (100-500 ng/µl) 1 µl
- Add primer (3 pmol/µl) 1 µl
- Add H2O to 10 µl
Cycle the reaction according to the following protocol:
30 seconds, 96°C => 15 seconds, 50°C => 4 minutes, 60°C => Cycle 25 times.

Notes:
Other cycle conditions may work well for individual users. These conditions work well for most samples processed in our labs using reduced volume and reduced terminator mix concentrations.
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