BigDye® Terminator 5X Sequencing Buffer

BigDye® Terminator 5X Sequencing Buffer
Description

The BigDye® Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or read length. Its buffer system enables to use less dye terminator mix.
           
MSDS          
MSDS          
 
Name Cat# Description Price Qty
BigDye Terminator 5X Sequencing Buffer
SBUF-100
1 ml
$25.00
BigDye Terminator 5X Sequencing Buffer
SBUF-110
28 ml
$470.00
BigDye Terminator 5X Sequencing Buffer
SBUF-120
233 ml
$3495.00
BigDye Terminator 5X Sequencing Buffer
SBUF-OEM
Any size
please inquire
Description:
The BigDye® Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or read length. Its buffer system enables to use less dye terminator mix.

Application:
DNA sequencing
Fragment analysis

Recommended Storage Conditions: -20°C

Recommended Protocol:
Prepare sequencing reactions (10µl rxn) according to the following:
- Add dye terminator mix 0.1-0.2µl
- Add 5X Sequencing Buffer 2µl
- Add template (100-500 ng/µl) 1 µl
- Add primer (3 pmol/µl) 1 µl
- Add H2O to 10 µl
Cycle the reaction according to the following protocol:
30 seconds, 96°C => 15 seconds, 50°C => 4 minutes, 60°C => Cycle 25 times.

Notes:
Other cycle conditions may work well for individual users. These conditions work well for most samples processed in our labs using reduced volume and reduced terminator mix concentrations.
There is no supporting data for this product. Will be available soon!!
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