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Blunting Express Kit
Blunting Express Kit

The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLAB’s Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B).  Our proprietary enzyme mix effectively fills in 5’ overhangs and eliminates A-tailed 3’ overhangs on the template.
 
Name Cat# Description Price Qty
Blunting Express Kit
BLEK-100
20 rxn
$75.00
Blunting Express Kit
BLEK-200
50 rxn
$175.00
Blunting Express Kit
BLEK-300
100 rxn
$300.00

Description:
The Blunting Express Kit can be used to generate blunt-ended DNA fragments for subsequent use in ligation, cloning, cDNA construction, and MCLABs Topomize Amplicon Library Prep Kit (Cat# TopoA-50A or TopoA-50B).  Our proprietary enzyme mix effectively fills in 5 overhangs and eliminates A-tailed 3 overhangs on the template. This Blunting Express Kit is optimized to provide a rapid workflow with only 2 minutes of incubation. Efficiency is combined with a high performance to streamline your blunt-end objectives.

Features:
Due to our proprietary Enzyme Mix, this kit provides a rapid workflow with a blunting step that requires only two minute incubation.  Its high efficiency is combined with a high performance to streamline the blunt end cloning objectives.

Recommended Storage Condition: -20°C

Kit Components:

  1. 10x Buffer
  2. dNTP Mix (25mM)
  3. Enzyme Mix

Figure:
PCR fragment size checked before and after end blunting by the kit. Data are generated by Applied Biosystems 3730/3730xl DNA Analyzer.

blunting express kit

Manual & Protocols

The Q&A for this product will be available soon.
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