Bst DNA Polymerase (large fragment)
Bst DNA Polymerase (large fragment)

For DNA sequencing high GC regions and rapid sequencing from nanogram amounts of DNA templates.
Name Cat# Size Concentration Price Qty
Bst DNA Polymerase (large fragment)
8,000 units
8 U/µl
Bst DNA Polymerase (large fragment)
10,000 units
100 U/µl
Bst DNA Polymerase (large fragment)
50,000 units
100 U/µl
Bst DNA Polymerase (large fragment)
100,000 units
100 U/µl
Bst DNA Polymerase (large fragment)
1,000,000 units
100 U/µl
Bst DNA Polymerase (large fragment)
200,000 units
400 U/µl
Bst DNA Polymerase (large fragment)
1,000,000 units
1,000 U/µl
Bst DNA Polymerase (large fragment)
Any Size
please inquire

A recombinant E. coli strain carrying the BST DNA Polymerase gene (large fragment).

Figure: MCLAB Bst DNA Polymerase (large fragment) shows higher strand -displacing polymerase activity than two other vendors' products.

Sequencing through problematic secondary structures

Bst DNA Polymerase (large fragment) is a portion of Bacillus stearothermophilus DNA Polymerase protein that contains 5´-> 3´ polymerase activity, but lacks 5'->3' and 3'->5' exonuclease activities.

Specific Activity: 120,000 U/mg

Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10X PCR buffer II

10x PCR Buffer ll:
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO4
1.0% Triton X-100
pH 8.8 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.

Recommended Storage Condition: -20°C

Kiefer, et al. Structure 15 January 1997. 5, 95-108.

1. Does Bst DNA Polymerase have a 3'->5' proofreading exonuclease activity?

2.What is the difference between Bst DNA Polymerase (regular) and Bst DNA Polymerase, large fragment?
Bst DNA Polymerase, large fragment does not have the N-terminal exonuclease domain, so it not only lacks 3'->5' exonuclease activity, it also lacks 5'->3' exonuclease activity.

3. What is the best temperature range for Bst DNA Polymerase to be used?
The best temperature range is 60-65°C, it has 100% activity. The enzyme also has activity  

4.  What are the benefits of choosing Bst DNA Polymerase?
Bst DNA Polymerase, either regular or large fragment, is good at strand displacement with optimal temperature of 60-65°. This gives researchers a wider range of reaction conditions to optimize strand displacement and primer annealing. This is useful in the design of sequencing strategies as well as isothermal amplification technologies. The elevated reaction temperature facilitates sequencing through GC rich regions.

5. Can Bst DNA Polymerase be used for thermal cycle sequencing?
No, the reaction temperatures are too high for enzyme stability. It becomes inactive at 80°C for 10 minutes.

BL21(DE3) Competent <i>E. coli</i>
HB101 Competent <i>E. coli</i>
BL21(DE3)pLysS Competent <i>E. coli</i>
BL21(DE3) Competent E. coli HB101 Competent E. coli BL21(DE3)pLysS Competent E. coli
Dh5-Alpha Competent  <em>E. coli </em>
Lyse-load solution (5x1ml)
T4 Endonuclease VII
Dh5-Alpha Competent E. coli Lyse-load solution (5x1ml) T4 Endonuclease VII
Customized Competent <i>E. coli</i>
2X HotStart PCR Master Mix
Exonuclease I (<i>E. coli</i>)
Customized Competent E. coli 2X HotStart PCR Master Mix Exonuclease I (E. coli)