Bst DNA Polymerase (regular)
Bst DNA Polymerase (regular)

For replicating difficult templates in various applications.
Name Cat# Size Concentration Price Qty
Bst DNA Polymerase (regular)
8,000 units, 8 U/µl
Bst DNA Polymerase (regular)
20,000 units, 8 U/µl
Bst DNA Polymerase (regular)
50,000 units, 8 U/µl
Bst DNA Polymerase (regular)
Any Size
please inquire

rBst DNA Polymerase is the product of the DNA poll gene of the thermophilic bacterium Bacillus stearothermophilus (Bst). As an enzyme, it contains the 5´ -> 3´ polymerase activity, but lacks the 5´ -> 3´ exonuclease domain. It also has optimal activity at 65°C and can be used to synthesize DNA in regions that containing template secondary structure or high GCs where other non-thermostable DNA polymerases may fail in sequencing. Therefore, rBst DNA Polymerase is useful in replicating difficult templates in various applications.

- Isothermal DNA amplification by the method of:
· loop-mediated isothermal amplification (LAMP)
· whole genome amplification (WGA)
· ramification amplification (RAM)
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA

An E. coli strain that contains the gene from Bacillus stearothermophilus.

Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10X PCR buffer II

10x PCR Buffer ll:
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO4
1.0% Triton X-100
pH 8.8 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.

Recommended Storage Condition: -20°C

Kiefer, et al. Structure 15 January 1997. 5, 95-108.

Manual & Protocols

COA ( Certificate of Analysis )

MSDS & Certificates

There is no supporting data for this product. Will be available soon!!
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