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Description: rBst DNA Polymerase is the product of the DNA poll gene of the thermophilic bacterium Bacillus stearothermophilus (Bst). As an enzyme, it contains the 5´ -> 3´ polymerase activity, but lacks the 5´ -> 3´ exonuclease domain. It also has optimal activity at 65°C and can be used to synthesize DNA in regions that containing template secondary structure or high GCs where other non-thermostable DNA polymerases may fail in sequencing. Therefore, rBst DNA Polymerase is useful in replicating difficult templates in various applications. Application: - Isothermal DNA amplification by the method of: · loop-mediated isothermal amplification (LAMP) · whole genome amplification (WGA) · ramification amplification (RAM) - Random-primed DNA labeling - Labeling by fill-in 5'-overhangs of dsDNA Source: An E. coli strain that contains the gene from Bacillus stearothermophilus. Supplied in: 10 mM Tris-HCL 50 mM KCl 1.0 mM Dithiothreitol 0.1 mM EDTA 0.1% Triton X-100 50% Glycerol pH 7.5 @ 25°C Supplied with: 10x PCR Buffer II 10x PCR Buffer ll: 200 mM Tris-HCL 100 mM Ammonium Sulfate 100 mM KCl 20 mM MgSO4 1.0% Triton X-100 pH 8.8 @ 25°C Unit Definition: 1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C. Recommended Storage Conditions: -20°C Reference: Kiefer, et al. Structure 15 January 1997. 5, 95-108. |
