This linearized vector dose not carry any tag sequence. The Cloning / expression region of the coding strand is shown in the PDF links. Sequencing primers for final confirmation is CMV promoter or T7 promoter and SV40 poly A reverse or SP6 promoter.
Choo-Choo PCR Cloning Kits ®is a highly efficient directional PCR cloning kits for rapid, ligase- and restriction enzyme-independent PCR cloning Kits.
