MCLAB Products

Competent E. Coli BL21(DE3)pLysS

Description

High yields, high efficiency, no heat shock necessary.

*Note:

Name	Cat#	Description	Price	Qty
BP-100 Kit	BP-100	1 x 10^8, 10 x 100µl	$110.00
BP-196 Kit	BP-196	1 x 10^8, 96 x 50µl	$275.00

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Competent E. Coli. BL21(DE3)pLysS
High yields, high efficiency, no heat shocknecessary. For Research Use Only.

Application

Ideal for expressing proteins that are toxic to E.Coli.
BL21(DE3)pLysS cells carry the lambda DE3 lysogen. In addition, L21(DE3)pLysS cells contain the pLysS plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. This provides tight control of T7 RNA polymerase,which is necessary when the recombinant protein to be expressed is toxic.

Recommended torage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions

Mix DNA with competent cell
Let stand on bench for 5 minutes
Direct plate

Genotype
F^-ompT hsdS_B(r_B^-m_B^-)gal dcm (DE3) pLysS (Cam^R)

Reference
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

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Competent E. Coli BL21(DE3)pLysS

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