Competent E. Coli BL21(DE3)

Competent E. Coli BL21(DE3)
Description

High yields, high efficiency, no heat shock necessary.
 
Name Cat# Description Price Qty
BS-100 Kit
BS-100
1 x 10^8, 10 x 100µl
$110.00
BS-196 Kit
BS-196
1 x 10^8, 96 x 50µl
$275.00
Competent E. Coli. BL21(DE3)

Application
  • Ideal for expressing proteins in E.Coli.
  • BL21(DE3) cells carry the lambda DE3 lysogen.
Recommended torage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions
  • Mix DNA with competent cell
  • Let stand on bench for 5 minutes
  • Direct plate
Genotype
F-ompT hsdSB(rB-mB-)gal dcm (DE3)

Reference
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.
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