Competent E. Coli DH5a

Description

DH5a™ is the most frequently used E.Coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a™ increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

*Note:

Name	Cat#	Description	Price	Qty
DA-100 Kit	DA-100	>1 x 10^9, 20 x 50µl	$110.00
DA-196 Kit	DA-196	>1 x 10^9, 96 x 50µl	$299.00
144 DA-100 Kits	DA-144A*	Pre-payment for one year, 15 plates of DA-196 Kit, individual shipping and handling charges will apply.	$3500.00

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Description:
DH5a™ is the most frequently used E.Coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a™ increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Application:

Highest transformation efficiency
General cloning
Blue-white selection
Plasmid isolation

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Genotype:
F- 80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17(rk-, mk+) phoAsupE44 -thi-1 gyrA96 relA1

Reference:
Woodcock, D.M., Crowther, D.M., Doherty, J., Jefferson,S., DeCruz, E., Noyer-Weidner, M., Smith, S.S., Michael, M.Z., and Graham, M.W., Nucl. Acids Res. (1989) 17, 3469-3478

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Competent E. Coli DH5a

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