Competent E. Coli. JM109
High stability, high efficiency, no heat shock necessary. For Research Use Only.
Application
- Ideal for sub-cloning, single strained DNA, high quality plasmid preparation, and library construction
- JM109 is a K strain bacterium that provides minimized recombination and aids in plasmid stability which results in high quality plasmid DNA preparation. The strain carries the hsdR17 genotype, which prevents cleavage of heter-ologous DNA by an endogenous endonuclease. JM109 strain supports Alpha-Complementation for blue/white screening for recombinant plasmids. The presence of the F' factor also allows growth of bacterio-phage M13 vectors for rescue of single stranded DNA. Transform efficiency is around 1.0 x 107 ~ 1.0 x 108 cfu/µg with pUC18 control DNA.
Recommended storage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions
- Mix DNA with competent cell
- Let stand on bench for 5 minutes
- Direct plate
Genotype
F' (traD36, proAB+, lacIq, lacZ-M15),endA1, recA1, hsdR17, (rk-, mk+), mcrA, supE44, e-gyrA96, relA1, -(lac-proAB)
Reference
- Yanisch-Perron, C.; Vieira, J.; and Messing, J. Gene, (1985) 33, 103-119
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