Csp68KVI

Recognition site:
CG^CG

Conditions for 100% Activity
1X Buffer R:
10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/ml BSA.
Incubate at 37°C.

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Typical ligation/recut assay results:
After 50-fold overdigestion with Bsh1236I, more than 95% of the DNA fragments can be ligated and recut.

Recommended storage condition:
-20°C.

Isoschizomers
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