DNA Polymerase I

Placeholder DNA Homopolymeric Tailing Super Mix Kit
Placeholder DNA polymerase, thermotoga neapolitana

$0.00 – $455.47

DNA Polymerase I is a DNA-dependent DNA polymerase. The enzyme catalyzes 5′ -> 3′ synthesis of DNA and exhibits 3′ -> 5’ exonuclease proofreading activity.
SKU: DPI Categories: ,
SKU OPTIONS PriceQuantity
DPI-100 2,500 units (10,000 U/ml) $151.82
✓
DPI-200 10,000 units (10,000 U/ml) $455.47
✓
DPI-OEM Any Size Please inquire
✓
Bulk Orders Add selected to cart ✓

Description

Description:
DNA Polymerase I is a mesophilic DNA polymerase that exhibits 5′ -> 3′ DNA synthesis activity in addition to both 3′ -> 5′ and 5′ -> 3′ exonuclease activities. The combination of DNA synthesis and 5′ -> 3′ nuclease characteristics enable nick-translation during DNA synthesis.

Applications:

  • Nick translation of DNA to obtain probes with a high specific activity
  • Second strand synthesis of cDNA

Source:
A recombinant E. coli strain carrying the PolA gene

Specific Activity: 6,850U/mg

Supplied in:
25 mM Tris-HCl
0.1 mM EDTA
1.0 mM DTTl
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x Blue Buffer

10x Blue Buffer:
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.

Recommended Storage Condition: -20°C

Additional information

OPTIONS

2,500 units (10,000 U/ml), 10,000 units (10,000 U/ml), Any Size

Manual & Protocols

 

COA ( Certificate of Analysis )

 

MSDS & Certificates

The Q&A for this product will be available soon.