DNA Size Standard

• Designed for ABI Genetic Analyzers: 310, 3100, 3130/3130XL and 3730/3730XL

Descriptions

MCLAB's DNA Size Standard series products are internal lane standards that are intended to be used in assigning sizes to DNA fragments on fluorescence-detecting instruments. The common applications include genotyping and DNA fragment Analysis. Each of these standards consists of 15 DNA fragments, ranging in 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490 and 500 bp. Each band is single-stranded and fluorescence-labeled either with carboxy-x-rhodamine (Rox dye) or MCLAB's proprietary fifth orange dye. Size fragments are evenly distributed and can be used for very accurate size calling.

• Red DNA Size Standard: has been adapted on DNA fragment analysis software, e.g. GeneMapper (ABI) and GeneScan (ABI) by using same parameters as ABI's GeneScan 500 ROX Size Standard.
  
• Orange DNA Size Standard: can be used at the same setting as of ABI's GeneScan 500 Liz Size Standard.
  
• Double Peak DNA Size Standard: similar to Red/Orange DNA Size Standard, except every fragment is accompanied by an one-base-plus band. For example, 50 becomes 50 and 51, 75 become 75 and76, etc. This single base separation is very useful for monitoring the performance of the installed capillary arrays.
  

Product Formats:

• (1) Normal (DSMR-100, DSMO-100, DSMD-100)
• (2) Premixed in Super-DI" Formamide (DSMR-101, DSMO-101, DSMD-101)
  

Recommended Loading

• (1) Normal: 0.5µl per well
• (2) Premixed in Super-DI" Formamide: 15µl per well
  

The premixed size standard products are ready-to-use and can be aliquoted into the plate well directly.

Storage Conditions

• Stable at 4°C or -20°C for 6 months
• Do not repeatedly freeze-thawing

Examples