Dh5-Alpha Competent E. Coli

Description:
DH5a is the most frequently used E.Coli strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Application:

• Highest transformation efficiency
• General cloning
• Blue-white selection
• Plasmid isolation

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Genotype:
F- 80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17(rk-, mk+) phoAsupE44 -thi-1 gyrA96 relA1

Reference:
Woodcock, D.M., Crowther, D.M., Doherty, J., Jefferson,S., DeCruz, E., Noyer-Weidner, M., Smith, S.S., Michael, M.Z., and Graham, M.W., Nucl. Acids Res. (1989) 17, 3469-3478
Competent Cell Type: Chemically

Transformation Efficiency (cfu/μg):
>1x10⁹

Blue-Whiet Screening:
Yes

Strain:
K12

Reduces Recombination:
Yes

Cloning Methylated DNA:
No

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Not Suitable

T1 Phage Resistant:
No

RecA Deficient:
Yes