<i>E. coli</i> SSB
E. coli SSB

Single-Stranded DNA Binding Protein (SSB) preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits which protects 8-16 nucleotides.
 
Name Cat# Size Concentration Price Qty
E.coli SSB
ESSB-100
1.25 mg
5.0 mg/ml
$125.00
E.coli SSB
ESSB-200
5.0 mg
5.0 mg/ml
$400.00
E.coli SSB
ESSB-OEM
Any Size
please inquire

Description:
Single-Stranded DNA Binding Protein (SSB) preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits which protects 8-16 nucleotides, while not binding well to double-stranded DNA. In nature, SSB participates in DNA replication, recombination, and repair functions. In vitro, SSB has been found to stimulate certain DNA polymerase-mediated reactions by relaxing DNA secondary structure and enhancing enzyme processivity.

Application:
- effective in fluorescence polarization assays
- eliminates pausing when sequencing through strong secondary structures
- helps obtain longer read lengths in pyrosequencing for SNP analysis

Source:
A recombinant E. coli strain carrying the E. coli SSB gene.

Supplied in:
50 mM Tris-HCl
200 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Recommended Storage Condition: -20°C

Manual & Protocols



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