Description:
Single-Stranded DNA Binding Protein (SSB) preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits which protects 8-16 nucleotides, while not binding well to double-stranded DNA. In nature, SSB participates in DNA replication, recombination, and repair functions. In vitro, SSB has been found to stimulate certain DNA polymerase-mediated reactions by relaxing DNA secondary structure and enhancing enzyme processivity.

Applications:

• Effective in fluorescence polarization assays
• Eliminates pausing when sequencing through strong secondary structures
• Helps obtain longer read lengths in pyrosequencing for SNP analysis

Source:
A recombinant E. coli strain carrying the E. coli SSB gene

Supplied in:
50 mM Tris-HCl
200 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Recommended Storage Condition: -20°C