EcoRI

Description

Recognition site: G^AATTC

Synonyms:
EcoRI, FunII

Source:
Purified from a strain of E. coli that bears the cloned EcoR I gene.

Supplied In:
50 mM Tris-HCL(pH 7.2)
300 mM NaCl
5 mM EGTA
0.5 mM EDTA
5 mM 2-mercaptoethanol
0.5 mg/ml BSA
50% (v/v) Glycerol
0.2% (w/v) Triton X-100

Reaction Condition:
Buffer at 37ºC

10x EcoR I Buffer
1M Tris-HCl
500 mM NaCl
100 mM MgCl₂
0.25% Triton® X-100
pH 7.5 @ 25°C

Typical Ligation/Recut Assay Results:
EcoR I –cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by EcoR I.

Recommended Storage Condition: -20 ºC.

Comments:
Does not cleave DNA when either the 3′ A or the C residue is N6-methyladenine or 5-methylcytosine, respectively. Partially resistant to heat inactivation (10 minutes, 65 ºC). Glycerol concentrations >5% (v/v), low-salt, or high-pH conditions may alter specificity.

Additional information

Manual & Protocols

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