Description
A class II apurinic/apyrimidic (AP) enzyme that cleaves 5’ to an AP site by hydrolysis leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus
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Description: Endonuclease IV (E. coli) is a class II apurinic/apyrimidic (AP) enzyme that cleaves 5' to an AP site by hydrolysis, leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5'-terminus. Endo IV can be used in vivo to repair free radical damage in DNA. Application: - DNA structure research - Studies of DNA damage and repair - Single cell electrophoresis (comet assay) - Anti-tumor drug studies Source: E. coli cells with a cloned nfo gene. Unit Definition: One unit of the enzyme relaxes 1µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 min at 37°C Storage Buffer: Supplied in 50% Glycercol, 50 mM Tris-HCL(pH 7.5), 1mM DTT, 0.1 M NaCl, 0.1% Triton X-100 Quality Control: Endonuclease IV is tested in degradation of dsDNA and is free of detectable RNase and double-stranded exonuclease activities. Recommended Storage Conditions: -20°C Reference: 1. Izumi, T. et al. (1992) J. Bacteriol. 174, 7711. 2. Levin, J.D. and Demple, B. (1996) Nucleic Acids Res. 24, 885. |
