Endonuclease IV, E. coli

Description:
Endonuclease IV, E. coli is a class II apurinic/apyrimidic (AP) enzyme that cleaves 5’ to an AP site by hydrolysis leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. Endo IV can be used in vivo to repair free radical damage in DNA.

Applications:

• DNA structure research
• Studies of DNA damage and repair
• Single cell electrophoresis (comet assay)
• Anti-tumor drug studies

Source:
E.coli cells with a cloned nfo gene.

Unit Definition:
One unit of the enzyme relaxes 1 µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 min at 37°C

Storage Buffer:
Supplied in 50% glycercol, 50 mM Tris-HCl (pH 7.5), 1mM DTT, 0.1 M NaCl, 0.1% Triton X-100

Quality Control:
Endonuclease IV is tested in degradation of dsDNA and is free of detectable RNase and double-stranded exonuclease activities.

Recommended Storage Conditions:
 -20°C

References:

1. Izumi, T. et al. (1992) J. Bacteriol. 174, 7711.
2. Levin, J.D. and Demple, B. (1996) Nucleic Acids Res. 24, 885.