New special offers are available!
Endonuclease IV, <i>E. coli</i>
Endonuclease IV, E. coli

A class II apurinic/apyrimidic (AP) enzyme that cleaves 5’ to an AP site by hydrolysis leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus
 
Name Cat# Size Concentration Price Qty
Endonuclease IV, E. coli
EIV-100
2,500 units
10 U/µl
$119.00
Endonuclease IV, E. coli
EIV-200
10,000 units
10 U/µl
$357.00
Endonuclease IV, E. coli
EIV-OEM
Any Size
please inquire

Description:
Endonuclease IV (E. coli) is a class II apurinic/apyrimidic (AP) enzyme that cleaves 5' to an AP site by hydrolysis, leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5'-terminus. Endo IV can be used in vivo to repair free radical damage in DNA.

Application:
- DNA structure research
- Studies of DNA damage and repair
- Single cell electrophoresis (comet assay)
- Anti-tumor drug studies

Source:
E. coli cells with a cloned nfo gene.

Unit Definition:
One unit of the enzyme relaxes 1µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 min at 37°C

Storage Buffer:
Supplied in 50% Glycercol, 50 mM Tris-HCl (pH 7.5), 1mM DTT, 0.1 M NaCl, 0.1% Triton X-100

10x Endonuclease IV Buffer
1M NaCl
500 mM Tris-HCl
100 mM MgCl2
10 mM DTT pH 7.9 @ 25°C

Quality Control:
Endonuclease IV is tested in degradation of dsDNA and is free of detectable RNase and double-stranded exonuclease activities.

Recommended Storage Condition: -20°C

Reference:
1. Izumi, T. et al. (1992) J. Bacteriol. 174, 7711.
2. Levin, J.D. and Demple, B. (1996) Nucleic Acids Res. 24, 885.

Manual & Protocols



MSDS & Certificates

The Q&A for this product will be available soon.
NGS Library Distribution Kit
Direct DNA Solution
2X MCAmp Library Amplification Master Mix
NGS Library Distribution Kit Direct DNA Solution 2X MCAmp Library Amplification Master Mix
$100.00
$400.00
$60.00
 
McExtract Gel Extraction Kit
dGTP BrightDye® Terminator Cycle Sequencing Kit
10X Thermal Chelator
McExtract Gel Extraction Kit dGTP BrightDye® Terminator Cycle Sequencing Kit 10X Thermal Chelator
$100.00
$500.00
$900.00
 
Ab Cloning 5'-RACE Kit
2X Ori-Master Plasmid Amplification Mix
Whole Genome Sequencing
Ab Cloning 5'-RACE Kit 2X Ori-Master Plasmid Amplification Mix Whole Genome Sequencing
$400.00
$199.00
$1000.00