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Epoxy-activated Agarose for Ligand or Protein Coupling
Epoxy-activated Agarose for Ligand or Protein Coupling

Epoxy-activated Agarose is a pre-activated affinity chromatography support for the immobilization of a variety of biomolecules that contain nucleophiles, such as hydroxyl, amino, or thiol groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA.
 
Name Cat# Description Price Qty
Epoxy-activated Agarose
EPA-50
15g epoxy-activated agarose CL-6B (semi-dry resin swelling to 50 ml drained medium)
$199.00
Epoxy-activated Agarose
EPA-250
75g epoxy-activated agarose CL-6B (semi-dry resin swelling to 250 ml drained medium)
$199.00

Description

Epoxy-activated Agarose is a pre-activated affinity chromatography support for the immobilization of a variety of biomolecules that contain nucleophiles, such as hydroxyl, amino, or thiol groups. These groups couple to the epoxy groups on the support, which is then used for the purification of proteins, carbohydrates, or DNA. 

Epoxy-activated Agarose CL-6B is based on highly cross-linked agarose 6% beads which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. The open pore structure allows coupling of large ligands for the purification of large targets, including protein A for purification of monoclonal antibodies, and other ligands for purification of recombinant proteins, such as those containing maltose-binding protein and calmodulin. In addition, Epoxy-activated Agarose CL-6B allows operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs. It is supplied as a semi-dry powder (1 g of powder gives ~3.3 ml final drained medium) to significantly shorten conditioning time before ligand coupling. 

Specifications

Chromatography technique Affinity coupling
Application Resin with a long hydrophilic spacer arm particular suitable for immobilization of small
Matrix molecules as well as proteins 
Active group Highly cross-linked 6% beaded agarose
Active group density >40 umol/ml drained medium
Spacer 1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage)  
Group to be coupled -OH, -NH2, or -SH
Bead geometry & size Spherical 50 to 150 um
Bead mean diameter d50v 90 um
Linear flow velocity <75 cm/h at 25 degC, HR 16/10 column, 5 cm bed height
Recommended linear flow rate <30 cm/h
Pressure/flow specification Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d.
pH Stability working range 3 to 13
pH Stability cleaning in place (cip) 2 to 14
Chemical stability Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane.
Storage 2 to 8 degC 
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