Firefly luciferase (photinus pyralis)
Firefly luciferase (photinus pyralis)

Firefly luciferase catalyses the oxidation of luciferin to oxyluciferin in an ATP-dependent process generating chemiluminescence at 560 nm (pH 7.8)
 
Name Cat# Size Concentration Price Qty
Firefly luciferase
FL0001
1mg
1 mg/ml
$85.00
Firefly luciferase
FL0002
2x1mg
1 mg/ml
$140.00
Firefly luciferase
FL00010
10x1mg
1 mg/ml
$420.00
Firefly luciferase
FL0OEM
Any Size
1 mg/ml
please inquire

Description:
Catalyses the oxidation of luciferin to oxyluciferin in an ATP-dependent process generating chemiluminescence at 560 nm (pH 7.8):

Firefly_luciferase.JPG

Applications:

  • Assesses the transcriptional activity in cells
  • Detects the level of cellular ATP in cell viability assays

Source:
E. coli recombinant enzyme prepared from a thermostable mutant.

Unit Definition:
One light unit produces a biometer peak height equivalent to 0.02 µCi of 14C in PPO/POPOP cocktail. Light units measured in 50 µl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.

Storage Conditions:
10 mM Tris-HCl pH 7.8
100 mM NaCl
0.1 mM DTT
0.1 mM EDTA
50% Glycerol

Heat Stability:
Active at 42°C for at least 24 hours (with < 5% loss of activity)

Recommended Storage Condition: -20°C

Reference:
Kricka UJ. (1988) "Clinical and biochemical applications of luciferases and luciferins", Anal Biochem. 175 (1): 14-21

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