Firefly luciferase (photinus pyralis)
$0.00 – $475.86
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| FL0001 | 1mg (1 mg/ml) | $96.31 | |||
| FL0002 | 2x1mg (1 mg/ml) | $158.62 | |||
| FL00010 | 10x1mg (1 mg/ml) | $475.86 | |||
| FL0OEM | Any Size (1 mg/ml) | Please inquire |
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Description
Description:
Catalyses the oxidation of luciferin to oxyluciferin in an ATP-dependent process generating chemiluminescence at 560 nm (pH 7.8):
Applications:
- Assesses the transcriptional activity in cells
- Detects the level of cellular ATP in cell viability assays
Source:
E. coli recombinant enzyme prepared from a thermostable mutant.
Unit Definition:
One light unit produces a biometer peak height equivalent to 0.02 µCi of 14C in PPO/POPOP cocktail. Light units measured in 50 µl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.
Storage Conditions:
10 mM Tris-HCl pH 7.8
100 mM NaCl
0.1 mM DTT
0.1 mM EDTA
50% Glycerol
Heat Stability:
Active at 42°C for at least 24 hours (with < 5% loss of activity)
Recommended Storage Condition: -20°C
Reference:
Kricka UJ. (1988) “Clinical and biochemical applications of luciferases and luciferins”, Anal Biochem. 175 (1): 14-21
Additional information
| OPTIONS | 1mg (1 mg/ml), 2x1mg (1 mg/ml), 10x1mg (1 mg/ml), Any Size (1 mg/ml) |
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