Firefly luciferase (photinus pyralis)

Description:
Description: catalyses the oxidation of luciferin to oxyluciferin in an ATP-dependent process generating chemiluminescence at 560 nm (pH 7.8):

Applications:

• Assess the transcriptional activity in cells
• Detect the level of cellular ATP in cell viability assays

Source:
E.coli recombinant enzyme prepared from a thermostable mutant.

Unit Definition:
One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C.

Buffer:
10% glycerol
50mM Tris-HCl
1 mM DTT
1 mM EDTA

Storage Conditions:
10mM Tris-HCl
PH 7.8
100mM NaCl
0.1mMDTT
0.1mM EDTA
50% glycerol

Heat Activation:
Active at 42°C for 24 hours (with < 5% loss of activity)

Recommended Storage Conditions:
-20°C

1. Kricka UJ. (1988) "Clinical and biochemical applications of luciferases and luciferins", Anal Biochem. 175 (1): 14-21