Description:
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA by reverse transcription (RT) at higher temperatures than the wild type M-MuLV to reach higher cDNA yields for difficult RNA transcriptions. This kit uses MCLAB's QuantumScript™ HD Reverse Transcriptase, a unique mutation with increased thermostability and reduced RNase H activity.
Applications:
-
cDNA Synthesis
-
RT-PCR
-
RT-qPCR
Components:
|
Components
|
Cap Color
|
Volume (50 rxn)
|
Volume (250 rxn)
|
|
5X RT Buffer
|
Red
|
125 μl
|
625 μl
|
|
RT Enzyme Mix
|
Clear
|
100 μl
|
500 μl
|
|
DTT
|
Yellow
|
50 μl
|
250 μl
|
|
dNTP
|
Blue
|
50 μl
|
250 μl
|
|
Oligo dT Primer
|
Green
|
50 μl
|
250 μl
|
|
Random Primer
|
Purple
|
50 μl
|
250 μl
|
Storage Condition:
-20 °C. It is recommended to reduce freeze-thaw cycles as few as possible.
Shipping Condition: Dry ice
|
