Fragmented DNA End-Repair Kit

Description

Description:
The MCLAB’s Fragmented DNA End Repair Kit is used for repairing fragmented DNA ends by sonication, nebulization or nucleases. The kit has been optimized to the maximum efficiency and convenience in DNA sample preparation workflow for next-generation sequencing (including Illumina^® Genomic DNA Sample Prep protocol, Roche 454^TM Library Preparation and Life Technologies SOLiD^TM Library Preparation).

Function:
The superior function of the Fragmented DNA End Repair Kit depends on MCLAB’s proprietary enzyme systems. Unique improvement to each key enzyme increases sensitivity, flexibility and speed to next-generation sequencing. The DNA fragment is converted to the blunt-ended DNA with a 5′-phosphate. DNA repaired by MCLAB’s Fragmented DNA End Repair Kit can be used directly for blunt end cloning or blunt-ended adaptor ligation, or be converted to DNA with 3´ dA-tail (MCLAB dA-Tailing kit, Cat #: NGDT-100) for subsequent ligation (MCLAB DNA Ligation kit, Cat #: NGDL-100).

Features:
– Simplified workflow, reduced hands-on time
– Optimized reaction conditions, increased efficiency
– Up to 10µg fragmented DNA
– Automation friendly format

Components:
Sufficient reagents are supplied in the Fragmented DNA End Repair Kit to convert 20 or 100 fragmented DNA samples to 5´-phosphorylated, blunt ended DNA.

Fragmented DNA End Repair Kit (20 reactions):
End Repair Enzyme Mix: 120µl
End Repair Buffer: 400µl

Fragmented DNA End Repair Kit (100 reactions):
End Repair Enzyme Mix: 600µl
End Repair Buffer: 2ml

Recommended Storage Condition: -20^oC

Additional information

Manual & Protocols

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