Hind III

Recognition site:
A^AGCTT

Source:
Purified from Haemophilus influenzae Rd.

Storage buffer:
10 mM Tris-HCl (pH 7.4), 200 mM NaCl, 0.5 mM EDTA, 1mM DTT, 0.5 mg/ml BSA, 50% (v/v) glycerol.

Reaction conditions:
Buffer 2 at 37°C.

Typical ligation/recut assay results:
Hind III-cleaved λ DNA is ≥95% ligated after 1 h. Of the ligated fragments, 100% are cleaved (recut) by Hind III.

Recommended storage conditions:
-20°C.

Comments:
Does not cleave DNA when either the 5’A or the C residue is N6 –methyladenine or 5-methylcytosine, respectively. Resistant to heat inactivation (10 min, 65°C).