MCLAB Products

HoTaq One-step Real-time RT-PCR Kit

Description

4x1.25ml for 200 reactions. Designed for all kinds of Real-time PCR machines.

*Note:

Name	Cat#	Description	Price	Qty
HoTaq One-step Real-time RT-PCR Kit	HTRT400	Regular level of ROX, for Real-time PCR Machines ABI 7000, 7300, 7700, 7900, 200 rnx, 4x1.25ml	$442.00
HoTaq One-step Real-time RT-PCR Kit	HTRT400LR	Low level of ROX, for Real-time PCR Machines ABI 7500, Mx 3000P, Mx 3005P, 200 rnx, 4x1.25ml	$442.00
HoTaq One-step Real-time RT-PCR Kit	HTRT400RF	ROX Free, for Real-time PCR Machines BioRad iCycler MiniOpticon, Opticon 2, Chromo4, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo4; Corbett Roto-gene 3000, 6000, 200 rnx, 4x1.25ml	$442.00

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HoTaq One-step Real-time RT-PCR Kit

Related Products: HTP, HSM

For Research Use Only.

PRODUCT
This is a high performance real-time PCR reagent. It utilizes our own proprietary quantitative PCR technology.

ADVANTAGES
1. MCLAB´s this product is similar to ABI´s TaqMan® One-Step RT-PCR Master Mix Reagents Kit (CAT# 4309169) but priced 25% less!

2. MCLAB´s 2X HotSybr PCR Reaction Mix products cuts down 1/2 total reaction time.

Normally the total reaction time is 4350 sec: 95°C, 10min → (95°C, 15sec→ 60°C ,60sec) x 50
For MCLAB´s 2X HotSybr PCR Reaction Mix, the total reaction time is 2350 sec: 95°C, 10min → (95°C, 5sec→ 60°C, 30sec) x 50

3. MCLAB's One-step RT-PCR products are faster.

4. MCLAB's 2X HoTaq PCR Reaction Mix products are superior in amplifying difficult templates.

This is the amplification of GPIIB gene (70% G+C) .
10 ~ 10K copies from 30pg human genomic DNA have been detected.

Applications
Probe based quantitative PCR including DNA quantification, 2-step RT PCR, SNP analysis, etc.

Primer and probe design
To achieve the best performance, appropriate software, such as ABI Primer Express^TM, should be used.Recommended reaction conditions:
55°C, 15min → 95°C, 10min. → (95°C, 5sec. → 60°C, 30sec.) for 50 cycles.
Supplied reverse transciptase is 200X.

Recommended storage condition
-20°C

Notes
To achieve accurate quantification, it is highly recommended to (1) avoid any RNase contamination; (2) design probe on sense strand; (3) use primer concentration from 100nM to 300nM; (4) shorten time between setting up reaction and loading plate onto PCR machine.

Reference

Holland, P. M., Abramson, R. D., Watson, R., and Gelfand, D. H. 1991. Proceedings of the National Academy of Sciences USA 88:7276-7280.
Livak, K. J., Flood, S. J. A., Marmaro, J., Giusti, W., and Deetz, K. 1995. PCR Methods and Applications 4:357-362.
Lee, L. G., Connell, C. R., and Bloch, W. 1993 Nucleic AcidsResearch 21:3761- 3766.

FOR RESEARCH USE ONLY.

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HoTaq One-step Real-time RT-PCR Kit

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