HoTaq DNA Polymerase (hot start)

Description:
HOTaq DNA Polymerase is hot-start Taq DNA Polymerase, which is a modified form of Taq DNA Polymerase. HOTaq DNA Polymerase is provided in an inactive state and has minimum enzymatic activity at ambient temperatures. This prevents the formation of misprimed products during reaction setup and the first denaturation step, leading to high PCR specific. It is suitable for diagnostic reaction without miner band. The enzyme is a highly processive 5’-> 3’ DNA polymerase that lacks 3’-> 5’ exonuclease activity. Each lot of HOTaq DNA polymerase is tested for PCR amplification.

The product is a chemically modified form of the Taq enzyme. It will be inactive at room temperature, and become active after 10 minutes at 94ºC.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus.(same as Taq DNA Polymerase)

• Amplification of DNA
• Sequencing ssDNA and dsDNA
• Site-directed mutagenesis

Comparison:
Here is the result of comparing MCLAB's HoTaq with some other leading brands.

Recommended Storage Conditions :
-20°C

Recommended Reaction Conditions
94°C, 10min. -> (94°C, 10sec. -> 55°C, 30sec. ->72°C, 30sec.) for 25 cycles.


Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

References:

1. Chien A, Edgar DB, Trela JM (1976). "Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus". J. Bact. 127 (3): 1550–7. PMC 232952. PMID 8432.
2. Saiki, RK; et al. (1988). "Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.". Science 239 (4839): 487–91. doi:10.1126/science.2448875. PMID 2448875.
3. Saiki, RK; et al. (1985). "Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia". Science 230 (4732): 1350–4. doi:10.1126/science.2999980. PMID 2999980.
4. Lawyer FC, et al. (1993). "High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase ...". PCR Methods Appl. 2 (4): 275–87. PMID 8324500.