HpyA V

Recognition Site:
CCTTC(6/5)

Source:
An E.coli strain that carries the cloned HpyAV gene from Helicobacter pylori 26695 (D.E.Berg).

Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Storage buffer:
10 mM Tris-HCl, 300 mM NaCl, 0.5 mM NiSO4, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C.

Reaction Conditions:
1X NEBuffer 4
Supplemented with 1 X Bovine Serum Albumin
Incubate at 37°C.

Ligation and Re-cutting:
After a 2-fold overdigestion with HpyAV, approximately 50% of the DNA fragments can be ligated with T4 DNA Ligase (at a 5' termini concentration of 1-2 μM) at 16ºC. Of these ligated fragments, approximately 50% can be recut with HpyAV.

Recommended storage condition:
-20°C.