Human Apoptosis PCR Array

Human Apoptosis PCR Array
Description

Apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis.
 
Name Cat# Description Price Qty
Human Apoptosis PCR Array
hap-102
96-well plate containing 88 pathway regulated genes plus 5 endogenous control genes, one non-transcribed genomic DNA contamination control, one reverse transcription control and one positive PCR control (2 plates)
$400.00
Human Apoptosis PCR Array
hap-104
96-well plate containing 88 pathway regulated genes plus 5 endogenous control genes, one non-transcribed genomic DNA contamination control, one reverse transcription control and one positive PCR control (4 plates)
$800.00
Human Apoptosis PCR Array
hap-102S
hap-102 with 2.5 ml SYBR Green master mix
$600.00
Human Apoptosis PCR Array
hap-104S
hap-104 with 5.0 ml SYBR Green master mix
$1200.00
Description:
Apoptosis is a genetically controlled mechanism of cell death involved in the regulation of tissue homeostasis. The 2 major pathways of apoptosis are the extrinsic (Fas and other TNFR superfamily members and ligands) and the intrinsic (mitochondria-associated) pathways, both of which are found in the cytoplasm. The extrinsic pathway is triggered by death receptor engagement, which initiates a signaling cascade mediated by caspase-8 activation. Caspase-8 both feeds directly into caspase-3 activation and stimulates the release of cytochrome c by the mitochondria. Caspase-3 activation leads to the degradation of cellular proteins necessary to maintain cell survival and integrity. The intrinsic pathway occurs when various apoptotic stimuli trigger the release of cytochrome c from the mitochondria (independently of caspase-8 activation). Cytochrome c interacts with Apaf-1 and caspase-9 to promote the activation of caspase-3. Recent studies point to the ER as a third subcellular compartment implicated in apoptotic execution. Alterations in Ca2+ homeostasis and accumulation of misfolded proteins in the ER cause ER stress. Prolonged ER stress can result in the activation of BAD and/or caspase-12, and execute apoptosis.

Gene List (88 genes):
ABL1;AIFM1; AKT1; AKT2; AKT3; APAF1; ATM; BAD; BAX; BCL2; BCL2L1; BID; BIRC2; BIRC3; CAPN1; CAPN2; CASP10; CASP3; CASP6; CASP7; CASP8; CASP9; CFLAR; CHP; CHP2; CHUK; CSF2RB; CYCS; DFFA; DFFB; ENDOD1; ENDOG; EXOG; FADD; FAS; FASLG; IKBKB; IKBKG; IL1A; IL1B; IL1R1; IL1RAP; IL3; IL3RA; IRAK1; IRAK2; IRAK3; IRAK4; MAP3K14; MYD88; NFKB1; NFKBIA; NGF; NTRK1; PIK3CA; PIK3CB; PIK3CD; PIK3CG; PIK3R1; PIK3R2; PIK3R3; PIK3R5; PPP3CA; PPP3CB; PPP3CC; PPP3R1; PPP3R2; PRKACA; PRKACB; PRKACG; PRKAR1A; PRKAR1B; PRKAR2A; PRKAR2B; PRKX; RELA; RIPK1; TNF; TNFRSF10A; TNFRSF10B; TNFRSF10C; TNFRSF10D; TNFRSF1A; TNFSF10; TP53; TRADD; TRAF2; XIAP

Reaction Conditions:
95°C, 10 minutes. -> (95°C, 5 seconds. -> 60°C, 30 seconds.) for 50 cycles.

Recommended Storage Conditions:-20°C

Notes:
To achieve accurate quantification, it is highly recommended to do replicates and to reduce pipetting error.
Apoptosis%20Signal_transduction_pathways

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

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