Human Cell Cycle PCR Array

Human Cell Cycle PCR Array

For Research Use Only.

Descriptions

The cell cycle, or cell-division cycle, is the series of events that takes place in a cell leading to its division and duplication. The human cell cycle is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cyclin-dependent kinases (CDKs) are key regulatory enzymes, each consisting of a catalytic CDK subunit and an activating cyclin subunit. CDKs regulate the cell's progression through the phases of the cell cycle by modulating the activity of key substrates. Downstream targets of CDKs include transcription factor E2F and its regulator Rb. Precise activation and inactivation of CDKs at specific points in the cell cycle are required for orderly cell division. Cyclin-CDK inhibitors (CKIs) are involved in the negative regulation of CDK activities, thus providing a pathway through which the cell cycle is negatively regulated.
    Human cells respond to DNA damage by activating signaling pathways that promote cell cycle arrest and DNA repair. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chek2, which in turn directly phosphorylates and activates p53 tumor suppressor protein. p53 and its transcriptional targets play an important role in both G1 and G2 checkpoints. ATR-Chk1-mediated protein degradation of Cdc25A protein phosphatase is also a mechanism conferring intra-S-phase checkpoint activation.

Gene List (88 genes)

ABL1; ANAPC1; ANAPC10; ANAPC11; ANAPC13; ANAPC2; ANAPC4; ANAPC5; ANAPC7; ATM; ATR; BUB1; BUB1B; BUB3; CCNA1; CCNA2; CCNB1; CCNB2; CCNB3; CCND1; CCND2; CCND3; CCNE1; CCNE2; CCNH; CDC14A; CDC14B; CDC16; CDC20; CDC23; CDC25A; CDC25B; CDC25C; CDC26; CDC27; CDC45L; CDC6; CDC7; CDK1; CDK2; CDK4; CDK6; CDK7; CDKN1A; CDKN1B; CDKN1C; CDKN2A; CDKN2B; CHEK1; CHEK2; CREBBP; CUL1; DBF4; E2F4; E2F5; EP300; ESPL1; FZR1; GADD45A; GADD45B; GADD45G; GSK3B; HDAC1; HDAC2; MAD1L1; MAD2L1; MAD2L2; MCM2; MCM3; MCM4; MCM5; MDM2; MYC; PCNA; PKMYT1; PLK1; PRKDC; RAD21; RB1; RBL1; RBL2; RBX1; SFN; SKP1; SKP2; TFDP1; TFDP2; TP53.

Reaction conditions
95°C, 10min. -> (95°C, 5 sec. -> 60°C, 30 sec.) for 50 cycles.

Storage Temperature: -20°C

Notes

To achieve accurate quantification, it is highly recommended to do replicates. Also it is important to reduce pipetting error.