MCLAB Products

Human and Mouse Housekeeping Gene Primer Sets

Description

4x1.25ml for 200 reactions. Designed for all kinds of real-time PCR machines;This is a high performance real-time PCR reagent. It utilizes our own proprietary quantitative PCR technology.



Protocol for human	Protocol for mouse

*Note:

Name	Cat#	Description	Price	Qty
Human and Mouse Housekeeping Gene Primer Sets	hHKG-100	2 human genes, h18S rRNA and hActß, 100rxns	$19.95
Human and Mouse Housekeeping Gene Primer Sets	hHKG-110	2 high expression level hACtß, hGapdh; 2 medium expression level genes, hHprt1, hTfrc; 2 low expression level genes, hGusb, hUbc; 100rxns	$49.95
Human and Mouse Housekeeping Gene Primer Sets	hHKG-120	All 12 human housing keeping genes at different expression levels, 100rxns	$79.95
Human and Mouse Housekeeping Gene Primer Sets	mHKG-100	2 mouse genes, m18S rRNA and mActß, 100rxns	$19.95
Human and Mouse Housekeeping Gene Primer Sets	mHKG-110	2 high expression level mACtß, mGapdh; 2 medium expression level genes, mHprt1, mHsp90ab1; 2 low expression level genes, mGusb, mTbp; 100rxns	$49.95
Human and Mouse Housekeeping Gene Primer Sets	mHKG-120	All 12 mouse housekeeping genes at different expression levels, 100rxns	$79.95

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Human and Mouse Housekeeping Gene Primer Sets

For Research Use Only.

Table: Validated primer sets for human and mouse housekeeping genes and their expression levels.

Human Genes			Mouse Genes
Name	GeneBank Accession #	Relative Expression*	Name	GeneBank Accession #	Relative Expression*
h18S rRNA	X0325	****	m18S rRNA	K01364	****
hActß	NM_001101	***	mAct²	NM_007393	***
hB2m	NM_004048	***	mB2m	NM_009735	***
hGapdh	NM_002046	***	mGapdh	NM_008084	***
hPpia	NM_021130	***	mPpia	NM_008907	***
hRpl13a	NM_012423	***	mRpl13a	NM_009438	***
hRplp0	NM_001002	**	mHsp90ab1	NM_008302	**
hHprt1	NM_000194	**	mLdhal6b	NM_175349	**
hTfrc	NM_003234	**	mHprt1	NM_013556	**
hHsp90ab1	NM_007355	*	mGusb	NM_010368	*
hGusb	NM_000181	*	mTbp	NM_013684	*
hUbc	NM_021009	*	mTfrc	NM_011638	*

* Summary of the expression levels of genes in comparison with 18S rRNA using our sensitivity human or mouse reference cDNA and our proprietary SYBR green master mix.

Related Products: qHRcDNA, HSM, HTP

Reaction conditions
95°C, 10 min -> (95°C, 15 Sec. -> 60°C, 30 sec.) for 40 cycles -> melting curve.

Storage Temperature: -20°C

Figure: Standard curve for housekeeping gene hprt1 expression at medium levels.

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Human and Mouse Housekeeping Gene Primer Sets

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