Human p53 signaling PCR Array

Human p53 signaling PCR Array

For Research Use Only.

Descriptions

p53 activation is induced by a number of stress signals, including DNA damage, oxidative stress and activated oncogenes. The p53 protein is employed as a transcriptional activator of p53-regulated genes. This results in three major outputs; cell cycle arrest, cellular senescence or apoptosis. Other p53-regulated gene functions communicate with adjacent cells, repair the damaged DNA or set up positive and negative feedback loops that enhance or attenuate the functions of the p53 protein and integrate these stress responses with other signal transduction pathways.

APAF1; ATM; ATR; BAI1; BAX; BBC3; BID; CASP3; CASP8; CASP9; CCNB1; CCNB2;CCNB3; CCND1; CCND2;CCND3; CCNE1; CCNE2; CCNG1; CCNG2; CD82; CDK1; CDK2; CDK4; CDK6; CDKN1A; CDKN2A; CHEK1; CHEK2; CYCS; DDB2; EI24; FAS; GADD45A; GADD45B; GADD45G; GTSE1; IGF1; IGFBP3; LRDD; MDM2; MDM4; PERP; PMAIP1; PPM1D; PTEN; RCHY1; RFWD2; RPRM; RRM2; RRM2B; SERPINB5; SERPINE1; SESN1; SESN2; SESN3; SFN; SHISA5; SIAH1; STEAP3; THBS1; TNFRSF10B; TP53; TP53AIP1; TP53I3; TP73; TSC2; ZMAT3.

Reaction conditions
95°C, 10min. -> (95°C, 5 sec. -> 60°C, 30 sec.) for 50 cycles.

Storage Temperature: -20°C

Notes

To achieve accurate quantification, it is highly recommended to do replicates. Also it is important to reduce pipetting error.