I-5™ Hi-Fi DNA Polymerase
I-5™ Hi-Fi DNA Polymerase

I-5 Hi-Fi DNA polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification from different templates including plasmids, BACS, genomic DNA, and lambda DNA.

 
Name Cat# Description Price Qty
I-5™ Hi-Fi DNA Polymerase
PDP-100
250 Units,2.5 U/μl
$147.50
I-5™ Hi-Fi DNA Polymerase
PDP-200
1,250 Units,2.5 U/μl
$572.50
I-5™ Hi-Fi DNA Polymerase
PDP-OEM
any size
please inquire

General Description
I-5 Hi-Fi DNA polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification of from different templates including plasmids, BACS, genomic DNA, and lambda DNA. It allows for amplification of up to 8kb with human genomic DNA and up to 21kb with lambda DNA. It has an extension speed of 1 kb / 10-15 seconds depending on template type. This allows the user to save time by speeding up PCR reactions and provides higher fidelity than Taq or Pfu.

Feature
• Robust – maximal success and minimal optimization needed
• Fidelity – 50X greater than Taq
• High Speed –10X faster than Pfu
• High Yield
• Versatile – best for long or difficult templates

Source
E. coli

Applications
PCR
Cloning
Long or Difficult Amplification
High-Throughput PCR

Supplied with
5X Reaction Buffer (1ml), 50mM MgCl2 (1ml)

Supplied in (buffer description)
20mM Tris-HCl, 100mM KCl, 1mM DTT, 0.1mM EDTA, 200ug/ml BSA, 50% glycerol, 1X stabilizer, pH 7.4 @ 25 C

Storage Condition
-20°C

Unit Definition
One unit is the amount of enzyme that incorporates 5 nmol of dNTPs into acid insoluble material in 15 minutes at 72°C.

References 
Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.
Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.

Shipping Condition
dry ice

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

The Q&A for this product will be available soon.
T4 Endonuclease VII
HB101 Competent <i>E. coli</i>
BigDye<sup>®</sup> Terminator 5X Sequencing Buffer
T4 Endonuclease VII HB101 Competent E. coli BigDye® Terminator 5X Sequencing Buffer
$200.00
$110.00
$25.00
 
JM109 Competent <i>E. coli</i>
Lyse-load solution (5x1ml)
BL21(DE3) Competent <i>E. coli</i>
JM109 Competent E. coli Lyse-load solution (5x1ml) BL21(DE3) Competent E. coli
$110.00
$95.00
$110.00
 
2X  HoTaq&trade; One-Step Real-Time RT-PCR Kit For TaqMan<sup>&reg;</sup>
2X HotStart PCR Master Mix
2X HotSybr qPCR Kit
2X HoTaq™ One-Step Real-Time RT-PCR Kit For TaqMan® 2X HotStart PCR Master Mix 2X HotSybr qPCR Kit
$50.00
$60.00
$100.00