
Also Available FastSep™ High Performance IDA Resins
Description FastSep high performance resins and chromatography cartridges - Numerous ligand-metal ion combinations Application - For His-tagged protein purification Purification of His-tagged Recombinant Proteins A his-tag, or polyhistidine tag, is a string of histidine residues at either the N or C terminus of a recombinant protein. Such a string can have 4 to 10 residues, with 6 being the most common a hexahistidine tag. Recombinant proteins can have more than one hexahistidine tag. How to choose different ligands and metal ions in IMAC? NTA and IDA are the two most widely used ligands in IMAC. NTA is a tetravalent ligand with coordination number 4 and therefore stronger coordination of metal ions, while IDA is a trivalent ligand with coordination number 3. Generally, NTA gives higher binding specificity and lower metal ion leaching, while IDA shows more non-specific binding and increased metal ion leaching. IDA can have higher metal ion loading density and usually requires much lower imidazole concentration in eluent.
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