IDA Agarose for His-tagged Recombinant Proteins (R.)

$0.00$2,264.87

Ni2+, Co2+, Cu2+, or Zn2+ charged iminodiacetic acid (IDA) coupled to Agarose CL-6B via a stable and uncharged long ether hydrophilic spacer arm, and offers high binding capacity and minimal non-specific binding.

Also Available FastSep™ High Performance IDA Cartridges

SKU OPTIONS PriceQuantity
NIIDA-25 Ni-IDA Resin, 25ml nickel-charged IDA resin (50% suspension, 50ml total volume) $180.15
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NIIDA-100 Ni-IDA Resin, 100ml nickel-charged IDA resin (50% suspension, 200ml total volume) $565.37
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NIIDA-500 Ni-IDA Resin, 500ml nickel-charged IDA resin (50% suspension, 1000ml total volume) $2,264.87
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COIDA-25 Co-IDA Resin, 25ml cobalt-charged IDA resin (50% suspension, 50ml total volume) $180.15
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COIDA-100 Co-IDA Resin, 100ml cobalt-charged IDA resin (50% suspension, 200ml total volume) $565.37
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COIDA-500 Co-IDA Resin, 500ml cobalt-charged IDA resin (50% suspension, 200ml total volume) $2,264.87
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CUIDA-25 Cu-IDA Resin, 25ml copper-charged IDA resin (50% suspension, 50ml total volume) $180.15
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CUIDA-100 Cu-IDA Resin, 100ml copper-charged IDA resin (50% suspension, 200ml total volume) $565.37
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CUIDA-500 Cu-IDA Resin, 500ml copper-charged IDA resin (50% suspension, 1000ml total volume) $2,264.87
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ZNIDA-25 Zn-IDA Resin, 25ml zinc-charged IDA resin (50% suspension, 50ml total volume) $180.15
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ZNIDA-100 Zn-IDA Resin, 100ml zinc-charged IDA resin (50% suspension, 200ml total volume) $565.37
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ZNIDA-500 Zn-IDA Resin, 500ml zinc-charged IDA resin (50% suspension, 1000ml total volume) $2,264.87
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XXIDA-OEM XX-IDA Resin, Any size any metal ion charged IDA resin Please inquire
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Description

Description

FastSep™ high performance resins and chromatography cartridges

– Numerous ligand-metal ion combinations
– High affinity & high capacity
– Minimal non-specific binding & excellent purity
– Long spacer arms & minimal steric hindrance
– Low metal ion leaching & excellent stability
– Maintain high performance after multiple repeated use

Application

– For His-tagged protein purification
– Simply replace your existing Ni-NTA resin or cartridge, no optimization or protocol changes necessary
– Purification under native and denaturing conditions
– Suitable for small proteins, large protein complexes, and proteins with low expression rates

Purification of His-tagged Recombinant Proteins

A his-tag, or polyhistidine tag, is a string of histidine residues at either the N or C terminus of a recombinant protein. Such a string can have 4 to 10 residues, with 6 being the most common — a hexahistidine tag. Recombinant proteins can have more than one hexahistidine tag.

In IMAC, metal ions are immobilized on a resin matrix using a chelating agent such as NTA or IDA. Ni2+, Co2+, Cu2+, and Zn2+ are the most commonly used ions for his-tag purification of a recombinant protein. The his-tag has a high affinity for these metal ions and binds strongly to IMAC column, while most other proteins in the lysate will not bind to resin, or bind only weakly. The use of a his-tag and IMAC can therefore provide relatively pure recombinant protein directly from a crude lysate.

How to choose different ligands and metal ions in IMAC?

NTA and IDA are the two most widely used ligands in IMAC. NTA is a tetravalent ligand with coordination number 4 and therefore stronger coordination of metal ions, while IDA is a trivalent ligand with coordination number 3. Generally, NTA gives higher binding specificity and lower metal ion leaching, while IDA shows more non-specific binding and increased metal ion leaching. IDA can have higher metal ion loading density and usually requires much lower imidazole concentration in eluent.

For metal ions going from Co2+, Zn2+, Ni2+, to Cu2+, generally binding affinity increases while binding specificity decreases. Cu2+ has the highest affinity giving the highest protein recovery, but low specificity. Co2+ has the lowest affinity but highest specificity, reducing the amount of impurity proteins in eluate. Ni2+ is often most widely used because it provides a good balance between affinity and specificity.

As many factors can affect the choice of optimal ligand-metal ion combination, the best approach is to try different combinations and let your proteins decide. FastSep(TM) high performance resins and cartridges offer numerous ligand-metal ion combinations to meet various purification needs.
FastSep(TM) high performance resins and chromatography cartridges are based on highly cross-linked agarose 6% beads which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. FastSep(TM) high performance resins and chromatography cartridges allow operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs.

Specifications

Chromatography technique Histidine-tagged protein purification
Matrix Highly cross-linked 6% beaded agarose
Active group Ni2+, Co2+, Cu2+, or Zn2+ charged Iminodiacetic Acid (IDA)
Active group density >40 umol/ml drained medium
Binding capacity >40 mg/ml drained medium
Spacer 12 atom stable and uncharged ether hydrophilic linkage
Bead geometry & size Spherical 50 to 150 um
Bead mean diameter d50v 90 um
Linear flow velocity <75 cm/h at 25 degC, HR 16/10 column, 5 cm bed height
Recommended linear flow rate <30 cm/h
Pressure/flow specification Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d.
1ml Cartridge bed dimensions 7×25 mm
1ml Cartridge bed height 25 mm
1ml Cartridge bed volume 1 ml
1ml Cartridge column I.D 7 mm
5ml Cartridge bed dimensions 16×25 mm
5ml Cartridge bed height  25 mm
5ml Cartridge bed volume 5 ml
5ml Cartridge column I.D 16 mm
Maximum cartridge flow rate 5 ml/min (1 ml cartridge) or 20 ml/min (5ml cartridge)
Recommended cartridge flow rate 1 ml/min (1 ml cartridge) or 5 ml/min (5 ml cartridge)
Maximum pressure during operation 5 bar [0.5 MPa] (70 psi)
pH Stability working range 3 to 13
pH Stability cleaning in place (cip) 2 to 14
Chemical stability Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane.
Storage 20% ethanol
Storage temperature 2 to 8 degC

Additional information

OPTIONS

Ni-IDA Resin, 25ml nickel-charged IDA resin (50% suspension, 50ml total volume), Ni-IDA Resin, 100ml nickel-charged IDA resin (50% suspension, 200ml total volume), Ni-IDA Resin, 500ml nickel-charged IDA resin (50% suspension, 1000ml total volume), Co-IDA Resin, 25ml cobalt-charged IDA resin (50% suspension, 50ml total volume), Co-IDA Resin, 100ml cobalt-charged IDA resin (50% suspension, 200ml total volume), Co-IDA Resin, 500ml cobalt-charged IDA resin (50% suspension, 200ml total volume), Cu-IDA Resin, 25ml copper-charged IDA resin (50% suspension, 50ml total volume), Cu-IDA Resin, 100ml copper-charged IDA resin (50% suspension, 200ml total volume), Cu-IDA Resin, 500ml copper-charged IDA resin (50% suspension, 1000ml total volume), Zn-IDA Resin, 25ml zinc-charged IDA resin (50% suspension, 50ml total volume), Zn-IDA Resin, 100ml zinc-charged IDA resin (50% suspension, 200ml total volume), Zn-IDA Resin, 500ml zinc-charged IDA resin (50% suspension, 1000ml total volume), XX-IDA Resin, Any size any metal ion charged IDA resin

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