IPTG, 1 M
IPTG, 1 M

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon (1).

 
Name Cat# Description Price Qty
IPTG, 1 M
IPTG-100
50 ml
$499.00
IPTG, 1 M
IPTG-200
100 ml
$899.00
IPTG, 1 M
IPTG-OEM
OEM
please inquire

Description:
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon (1).

Applications:

  • Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1)
  • Induction of gene expression under control of the lac promoter

Empirical Formula: C9H18O5S

Molecular Weight: 238.30

Recommended Storage Condition: -20oC. We recommend making single-use aliquots to prevent repeated freeze-thaw cycles of IPTG in solution.

Reference:
1. J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, A1.27, 2001) pp. 1.124-1.125.

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