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IgG purification using Protein G
IgG purification using Protein G

Recombinant Protein G is an immunoglobulin-binding protein derived from the cell wall of certain strains of b-hemolytic Streptococci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein G is a major application in purifying antibodies. Protein G is considered a universal reagent in biochemistry and immunology.
 
Name Cat# Description Price Qty
IgG purification using Protein G
AIPG-100
Starting Material Hybridoma medium, 100 ml hybridoma medium (2 – 5 mg)
$198.00
IgG purification using Protein G
AIPG-200
Starting Material Frozen cells, 5 mg purified antibody
$315.00
IgG purification using Protein G
AIPG-300
Starting Material Mouse ascites, 20 ml ascites
$513.00

Description:
Recombinant Protein G is an immunoglobulin-binding protein derived from the cell wall of certain strains of b-hemolytic Streptococci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein G is a major application in purifying antibodies. Protein G is considered a universal reagent in biochemistry and immunology.

Starting Material

Time

Products

Hybridoma medium

1-3 weeks

100 ml hybridoma medium (2 – 5 mg)

Frozen cells

1-3 weeks

5 mg purified antibody

Mouse ascites

1-3 weeks

20 ml ascites

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Prepacked Columns
Epoxy-activated Agarose for Ligand or Protein Coupling
 FastSep™ NTA Agarose for His-tagged Recombinant Proteins (R.)
Prepacked Columns Epoxy-activated Agarose for Ligand or Protein Coupling FastSep™ NTA Agarose for His-tagged Recombinant Proteins (R.)
$100.00
$100.00
$100.00
 
 FastSep™ NTA Agarose for His-tagged Recombinant Proteins (C.)
 FastSep™ IDA Agarose for His-tagged Recombinant Proteins  (C.)
 FastSep™ NTA Agarose for Phosphoproteins & Phosphopeptides  (C.)
FastSep™ NTA Agarose for His-tagged Recombinant Proteins (C.) FastSep™ IDA Agarose for His-tagged Recombinant Proteins (C.) FastSep™ NTA Agarose for Phosphoproteins & Phosphopeptides (C.)
$100.00
$100.00
$100.00
 
 FastSep™ NTA Agarose for Phosphoproteins & Phosphopeptides  (R.)
 FastSep™ IDA Agarose for Phosphoproteins & Phosphopeptides (R.)
 FastSep™ Metal Ion Free NTA or IDA Agarose (C.)
FastSep™ NTA Agarose for Phosphoproteins & Phosphopeptides (R.) FastSep™ IDA Agarose for Phosphoproteins & Phosphopeptides (R.) FastSep™ Metal Ion Free NTA or IDA Agarose (C.)
$100.00
$100.00
$100.00