Inorganic Pyrophosphatase, E. coli

Description:
Inorganic pyrophosphatase (PPase) is ubiquitous in nature and plays an important role in energy metabolism, providing a thermodynamic pull for biosynthetic reactions such as protein, RNA, and DNA synthesis. Escherichia coli K-12 gene ppa encoding inorganic pyrophosphatase (PPase) was cloned and sequenced. The 5' end of the ppa mRNA was identified by primer extension mapping.

Source:
E. Coli strain carrying a plasmid encoding pyrophosphatase from Escherichia coli K-12.

Applications:
-  role in protein, RNA, and DNA synthesis
-  catalyzing the reaction PPi + H2O → 2Pi

Unit Definition:
One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.

Recommended Storage Conditions:
-20°C

References:
Kornberg, A. 1962. On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions, p. 251-264. In H. Kasha and P. Pullman (ed.), Horizons in biochemistry. Academic Press, New York.