Inorganic Pyrophosphatase, yeast
Inorganic Pyrophosphatase, yeast

The Pyrophosphatase, inorganic (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates
 
Name Cat# Size Concentration Price Qty
Inorganic Pyrophosphatase, yeast
PI-100
25 units
100 U/ml
$96.00
Inorganic Pyrophosphatase, yeast
PI-200
100 units
100 U/ml
$288.00
Inorganic Pyrophosphatase, yeast
PI-OEM
Any Size
please inquire

Description:
The Pyrophosphatase, inorganic (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to two orthophosphates. The enzyme requires a divalent metal cation, with Mg2+ conferring the highest activity.

Application:
- High yield synthesis of RNA by in vitro transcription(1,2)
- DNA polymerization reactions: preventing accumulation of pyrophosphate(3, 4)
- Removal of contaminant PPi in reagents used for SNP genotyping by methods based on the detection of pyrophosphate(5)

Source:
E. coli cells with a cloned ppa gene of Sacharomyces cerevisiae.

Unit Definition:
One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCL, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 ml).

Storage Conditions:
20 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Recommended Storage Condition: -20°C

References:
1. Cooperman, B.S., The mechanism of action of yeast inorganic pyrophosphatase, Meth. Enzymol., 87, 526-548, 1982.
2. Cunningham, P.R. and Ofengand, J., Use of inorganic pyrophostase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase, Biotechniques, 9, 713-714, 1990.
3. Tabor, S., Richardson, C.C., DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Effect of pyrophosphorolysis and metal ions, J. Biol. Chem., 265, 8322-8328, 1990.
4. Dean, B.F., et al., Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed Rolling Circle amplification, Genome Res., 11, 1095-1099, 2001.
5. Zhou, G.H., et al., Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER), Nucleic Acids Res., 29, E93, 2001.



COA ( Certificate of Analysis )



MSDS & Certificates

The Q&A for this product will be available soon.
Exonuclease VII
5’ Adenylation (rApp Modification) Service
2X MCAmp Library Amplification Master Mix
Exonuclease VII 5’ Adenylation (rApp Modification) Service 2X MCAmp Library Amplification Master Mix
$120.00
$400.00
$60.00
 
DNA Fragment Size Analysis Kit
Human Trypsin 1
BrightDye® Terminator Cycle Sequencing Kit
DNA Fragment Size Analysis Kit Human Trypsin 1 BrightDye® Terminator Cycle Sequencing Kit
$100.00
$100.00
$500.00
 
Blunting Express Kit
5´ DNA  Adenylation Kit
2X HoTaq™ SYBR Green One-Step Real-Time RT-PCR Kit
Blunting Express Kit 5´ DNA Adenylation Kit 2X HoTaq™ SYBR Green One-Step Real-Time RT-PCR Kit
$50.00
$80.00
$50.00