Description
Modified Enzyme. catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
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Description: Lambda Exonuclease is a highly processive exonuclease which selectively degrades the 5'-phosphorylated strand of double-stranded DNA via the stepwise 5' to 3' release of mononucleotides from duplex DNA. Lambda Exonuclease is inactive against 5'-hydroxyl termini(1), and will not initiate excision at a nick or gap(2), though it will degrade a 5'-overhanging tail from duplex DNA at a greatly reduced rate. Specific Activity: 100,000 U/mg Application: Catalyzes the removal of 5'-mononucleotides from duplex DNA. Source: Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda. Supplied in: 25 mM Tris-HCL 50 mM NaCl 1.0 mM Dithiothreitol 0.1% mM EDTA 50% Glycerol pH 7.5 @ 25°C Supplied with: 10x Lambda Exo Reaction Buffer 10x Lambda Exo Reaction Buffer: 670 mM Glycine 25 mM MgCl2 pH 9.4 @ 25°C Unit Definition: One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrates in 30 minutes at 37°C. Recommended Storage Conditions: -20°C Reference: 1. Ausubel, F. M.,et al. (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.) 2. Little, J.W. (1981) Gene Amp. Anal., 2, 135-145 |
