Lambda Exonuclease

Lambda Exonuclease
Description

Modified Enzyme. catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
 
Name Cat# Size Concentration Price Qty
Lambda Exonuclease
LE-100
5,000 units
20,000 U/ml
$134.00
Lambda Exonuclease
LE-200
20,000 units
20,000 U/ml
$402.00
Lambda Exonuclease
LE-OEM
Any Size
please inquire
10x Lambda Exo Reaction Buffer
B-LE10
50 ml
$40.00
Description:
Lambda Exonuclease is a highly processive exonuclease which selectively degrades the 5'-phosphorylated strand of double-stranded DNA via the stepwise 5' to 3' release of mononucleotides from duplex DNA. Lambda Exonuclease is inactive against 5'-hydroxyl termini(1), and will not initiate excision at a nick or gap(2), though it will degrade a 5'-overhanging tail from duplex DNA at a greatly reduced rate.

Specific Activity: 100,000 U/mg

Application:
Catalyzes the removal of 5'-mononucleotides from duplex DNA.

Source:
Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.

Supplied in:
25 mM Tris-HCL
50 mM NaCl
1.0 mM Dithiothreitol
0.1% mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10x Lambda Exo Reaction Buffer

10x Lambda Exo Reaction Buffer:
670 mM Glycine
25 mM MgCl2
500 μg/ml BSA
pH 9.4 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrates in 30 minutes at 37°C.

Recommended Storage Conditions: -20°C

Reference:
1. Ausubel, F. M.,et al. (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.)
2. Little, J.W. (1981) Gene Amp. Anal., 2, 135-145

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

There is no supporting data for this product. Will be available soon!!
Ammonium Persulfate (APS)
Glucose Solution
Precast Agarose Gels  12 wells
Ammonium Persulfate (APS) Glucose Solution Precast Agarose Gels 12 wells
$65.00
 
Ori Master Mix
MCNext™ UT DNA Sample Prep Kit
Fpg, E. coli
Ori Master Mix MCNext™ UT DNA Sample Prep Kit Fpg, E. coli
$199.00
$90.00
$100.00
 
IPTG, 1 M
DEPC-Treated Water
Fragment Analysis Matrix
IPTG, 1 M DEPC-Treated Water Fragment Analysis Matrix
$40.00
$100.00

Customer feedback

There have been no reviews for this product.