Lambda Exonuclease
Lambda Exonuclease

Lambda Exonuclease catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
 
Name Cat# Size Concentration Price Qty
Lambda Exonuclease
LE-100
5,000 units
20,000 U/ml
$134.00
Lambda Exonuclease
LE-200
20,000 units
20,000 U/ml
$402.00
Lambda Exonuclease
LE-OEM
Any Size
please inquire

Description:
Lambda Exonuclease is a highly processive exonuclease which selectively degrades the 5'-phosphorylated strand of double-stranded DNA via the stepwise 5' to 3' release of mononucleotides from duplex DNA. Lambda Exonuclease is inactive against 5'-hydroxyl termini(1), and does not initiate excision at a nick or gap(2), though it degrades a 5'-overhanging tail from duplex DNA at a greatly reduced rate.

Specific Activity: 100,000 U/mg

Application:
Catalyzes the removal of 5'-mononucleotides from duplex DNA.

Source:
Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.

Supplied in:
25 mM Tris-HCl
50 mM NaCl
1.0 mM DTT
0.1% mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10x Lambda Exo Reaction Buffer

10x Lambda Exo Reaction Buffer:
670 mM Glycine
25 mM MgCl2
500 μg/ml BSA
pH 9.4 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrates in 30 minutes at 37°C.

Recommended Storage Condition: -20°C

References:
1. Ausubel, F. M.,et al. (1987) Current Protocols in Molecular Biology (John Wiley and Sons, Inc.)
2. Little, J.W. (1981) Gene Amp. Anal., 2, 135-145

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

The Q&A for this product will be available soon.
Recombinant Human BDNF
Agarose
NanoPOPô 10X Running Buffer (with EDTA)
Recombinant Human BDNF Agarose NanoPOPô 10X Running Buffer (with EDTA)
$100.00
$100.00
$88.00
 
MCMax Cell-Free DNA Extraction Kit
Uracil DNA Glycosylase (UDG)
200x Blue Stain
MCMax Cell-Free DNA Extraction Kit Uracil DNA Glycosylase (UDG) 200x Blue Stain
$400.00
$100.00
$20.00
 
Antibody Sequencing Service
7M Guanidine HCl Solution
Antibody Sequencing Service 7M Guanidine HCl Solution
$200.00
$150.00