DNA/RNA Ligases

DNA/RNA Ligases

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E.coli DNA ligase
E. coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of phosphodiester bonds between complementary 3´-hydroxyl and 5´-phosphoryl termini of dsDNA.
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T3 DNA Ligase
T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.
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T4 DNA Ligase
Modified enzyme. Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini of duplex DNA or RNA .
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T4 RNA Ligase 1 (ssRNA Ligase)
T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
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T4 RNA Ligase 2 (dsRNA Ligase)
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation.
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T4 RNA Ligase 2 (truncated) (RNL2)
MCLAB’s truncated  T4 RNA Ligase 2 was developed specifically for demanding Next-Generation RNA Sequencing applications.  The truncated  ligase 2 specifically ligates the adenylated 5´ end of an adapter to the 3´ end of RNA.. The enzyme does not require ATP for ligation but does need an adenylated substrate. By not having extra ATP in the reaction it will dramatically reduce the amount of ligation between random RNA molecules.
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T7 DNA Ligase
T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.
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Taq DNA Ligase
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor.
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